引用本文:	郇聘,张晓军,李富花,张洋,赵翠,刘保忠,相建海.栉孔扇贝热休克蛋白70（HSP70）基因的BAC-FISH 定位[J].海洋科学,2009,33(7):10-15.

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栉孔扇贝热休克蛋白70（HSP70）基因的BAC-FISH 定位
郇聘¹, 张晓军², 李富花², 张洋³, 赵翠¹, 刘保忠², 相建海²
1.中国科学院海洋研究所，中国科学院研究生院;2.中国科学院海洋研究所;3.中国科学院海洋研究所，TexasA&M University

摘要:

利用BAC-FISH技术，将包含HSP70基因的BAC克隆定位到栉孔扇贝的一对同源染色体的长臂上。HSP70基因的染色体定位将对深入研究该基因的结构及功能并将其应用于生产实践提供基础支持。同时，本研究是首次对栉孔扇贝低拷贝基因进行染色体定位的实践，其结果将为栉孔扇贝的染色体的深入研究以及染色体鉴别等工作提供必要的参考。

关键词: 栉孔扇贝热休克蛋白70 荧光原位杂交染色体

DOI：

分类号:

基金项目:国家自然科学基金重点资助项目（30730071）

Chromosomal localization of heat shock protein 70 (HSP70)

HUAN Pin,ZHANG Xiao-jun,LI Fu-hua,ZHANG Yang,ZHAO Cui,LIU Bao-zhong,Xiang Jian-hai

Abstract:

Zhikong scallop (Chlamys farreri) is one of key species in the marine aquaculture of China. Till present, the disease control is the main issue and many subjects were focused on disease- resistance and stress-related genes. Heat shock protein 70 (HSP70) shows diverse functions in the anti-stress reaction and pathogen-resistance reaction. However, the studies on HSP70 in C. farreri were mainly on a sequence analysis and expression profile, there is no result about the gene localization of HSP70. In this study, with the support of a BAC DNA library of C. farreri, a HSP70-containing BAC clone, CFB123C08, was mapped to the long arms of a pair of homologous chromosomes of C. farreri using BAC-FISH. This study would facilitate further studies on the HSP70 of C. farreri. And as the first attempt of chromosomal mapping of low-copy genes in C. farreri, this study would promote the researches on the chromosome identification of C. farreri.

Key words: Chlamys farreri HSP70 BAC-FISH Chromosome