引用本文:	黄晓荣,章龙珍,庄平,江琪,刘鉴毅,姚志峰,赵峰,张涛,冯广朋.超低温冷冻对长鳍篮子鱼精子中几种酶活性的影响[J].海洋科学,2009,33(7):16-22.

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超低温冷冻对长鳍篮子鱼精子中几种酶活性的影响
黄晓荣¹, 章龙珍¹, 庄平², 江琪¹, 刘鉴毅³, 姚志峰¹, 赵峰¹, 张涛³, 冯广朋¹
1.中国水产科学研究院东海水产研究所，上海海洋大学;2.中国水产科学研究院东海水产研究所，上海海洋大学，上海市高校水产养殖学E-研究院;3.中国水产科学研究院东海水产研究所

摘要:

研究超低温冷冻保存（?196℃）对长鳍篮子鱼（Siganus canaliculatus）精子内总ATP酶、肌酸激酶（CK）、琥珀酸脱氢酶（SDH）、乳酸脱氢酶（LDH）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）、谷胱甘肽过氧化物酶（GSH-PX）等酶活性的影响。运用试剂盒分别测定了冷冻前后长鳍篮子鱼精子内几种酶活性的变化。结果表明，经过超低温冷冻保存后，精子内GR活性显著升高（P<0.05），酶活性从冻前的163.54 U/L±17.14 U/L上升到239.33 U/L±22.24U/L；其余7种酶的活性均显著下降（P<0.05），超低温冷冻对长鳍篮子鱼精子内不同酶活性和精子活力均有较大影响。

关键词: 超低温冷冻长鳍篮子鱼（Siganus canaliculatus）精子酶活性

DOI：

分类号:

基金项目:国家科技支撑计划项目(2006BAD03B08-08)；国家科技基础条件平台项目(2006DKA30470_004)；中央级公益性科研院所基本科研业务费专项资金项目(2007T02)

Effects of cryopreservation on enzyme activity of Siganus canaliculatus spermatozoa

HUANG Xiao-rong,ZHANG Long-zhen,ZHUANG Ping,JIANG Qi,LIU Jian-yi,YAO Zhi-feng,ZHAO Feng,ZHANG Tao,FENG Guang-peng

Abstract:

This paper studied the effects of cryopreservation(-196℃) on the enzyme activity(total ATP, CK, SDH, LDH, SOD, CAT, GSH-PX and GR) in Siganus canaliculatus spermatozoa. The changes of enzyme activity were analysed in the spermatozoa using the reagent box respectively. The results showed the activities of spermatozoa dropped after cryopreservation, GR activities increased significantly(P<0.05), the activities added from 163.54 U/L±17.14 U/L to 239.33 U/L±22.24 U/L, other seven kinds of enzyme activities descended significantly(P<0.05). The activities of total ATP, CK, SDH dropped from 9.20 U/mL±0.28 U/mL,25.59 U/mL±0.13 U/mL and 18.33 U/mL±2.52 U/mL to 4.18 U/mL±0.81 U/mL,17.87 U/mL±0.56 U/mL and 9.33 U/mL±1.53 U/mL respectively, the activities of LDH, SOD and CAT declined from 14 828.66 U/L±866.56 U/L,421.68 U/mL±12.66 U/mL and 121.17 U/mL±15.04 U/mL to 11324.33 U/L±689.58 U/L,199.56 U/ml±50.55 U/ml and 74.60 U/mL±12.70 U/mL respectively. Activity of GSH-PX dropped from 55.22 U/(min·mL)±2.11 U/(min·mL) to 28.15 U/(min·mL)±1.78 U/(min·mL).Cryopreservation had a great effect on the enzyme activity and spermatozoa energy of Siganus canaliculatus.

Key words: cryopreservation Siganus canaliculatus spermatozoa enzyme activity