|TRAF是机体内一种具有信息传导作用的胞内因子, 承担多个受体家族的信号转导工作, 在固有免疫以及获得性免疫方面都发挥出重要作用。TRAF作为一类胞内接头蛋白, 对多条信号途径的活化具有一定的影响, 包括细胞的增殖、生存、凋亡、炎症反应、免疫反应等。目前已有研究表明鱼类中的TRAF基因也发挥重要的免疫防疫作用。以许氏平鲉为研究对象, 基于基因组和转录组的数据库, 进行了许氏平鲉TRAF基因家族的系统鉴定。在许氏平中共鉴定到9个TRAF基因, 并对这些TRAF基因的长度和氨基酸个数进行了统计分析。同时, 分析了这9个TRAF基因的结构特征。选取了包括许氏平鲉在内的6种鱼类进行了TRAF基因的共线性分析。结果显示, TRAF基因在硬骨鱼类中具有保守的共线性。通过系统发育分析, 更好地解析了TRAF家族基因的进化关系, 同时也证明了对其鉴定及命名的准确性。此外, 还进行了TRAF家族基因的蛋白质相互作用网络预测以及表达模式分析。通过蛋白质相互作用的网络分析, 得到TRAF基因与其互作蛋白的关联状况。在杀鱼爱德华氏菌侵染许氏平鲉后的不同时间点, 探究TRAF基因在杀鱼爱德华氏菌刺激下的表达模式。定量结果显示, 除TRAF4.1外, 其他TRAF基因在感染后均存在不同程度的上调。综上所述, 从分子水平上揭示了许氏平鲉TRAF家族基因的结构和功能, 揭示了TRAF可能依赖的免疫通路, 为进一步探究TRAF家族基因在许氏平先天免疫过程中的作用奠定了基础。
|关键词: 关键词TRAF 信号传导 表达模式 许氏平鲉 免疫应答
|IDENTIFICATION AND ANALYSIS OF TUMOR NECROSIS FACTOR RECEPTOR- ASSOCIATED FACTORS (TRAF) AND THEIR EXPRESSION PATTERN IN SEBASTES SCHLEGELII AFTER EDWARDSIELLA PISCICIDA INFECTION
LIU Xian-Tong, WANG Ning-Ning, WU Rui-Xue, LI Chao, CAO Min
School of Marine Science and engineering, Qingdao Agricultural University, Qingdao 266109, China
|Tumor cell necrosis factor receptor-related factor (tumor necrosis factor receptor-associated factors, TRAF) is an intracellular factor with information conduction effect in the body, which is responsible for the signal transduction efforts in many receptor families and plays an important role in both innate immunity and acquired immunity. As an intracellular adaptor protein, TRAF has a certain influence on the activation of multiple signaling pathways, including cell proliferation, survival and apoptosis participate in inflammatory and immune responses. At present, studies have shown that TRAF genes in fish also play an important role. Therefore, the whole genome of TRAF gene of Sebastes schlegelii was identified based on the database of genome and transcriptome. A total of nine TRAF genes were identified in S. schlegelii, and the length and number of amino acids of these TRAF genes were analyzed. Meanwhile, the structural characteristics of these nine TRAF genes were analyzed. Six species of fish including S. schlegelii were selected for the collinearity analysis of TRAF gene. Results show that the TRAF genes has conserved collinearity in bony fish. Through phylogenetic analysis, the evolutionary relationship of TRAF family genes was better revealed, and the accuracy of identification and nomenclature of TRAF family genes was confirmed. In addition, protein-protein interaction network prediction and expression pattern of TRAF family genes were analyzed. The expression patterns of TRAF genes stimulated by Edwardsiella piscicida at different time points in the infected S. schlegelii were studied. Results show that all the TRAF genes except TRAF4.1 were up-regulated in different degrees after infection. This study revealed the structure and function of TRAF family genes at molecular level, and the immune pathway that TRAF may depend on, laying a foundation for further exploring the role of TRAF family genes in the innate immunity of S. schlegelii.
|Key words: TRAF signaling transduction expression patterns Sebastes schlegelii immune response