| 摘要: |
| 采用鳗弧菌灭活疫苗对大菱鲆(Scophthalmus maximus)雌性亲鱼免疫方法, 进行了母源免疫对子代抗体水平、变化规律的影响及卵子中抗体蛋白空间分布特征的研究。结果表明, 免疫亲鱼产出的卵子中IgM抗体水平显著高于对照组。胚胎发育过程中, 抗体水平整体呈下降趋势, 且48h前下降迅速, 免疫组抗体水平显著高于对照组(P<0.05); 48h以后, 免疫组和对照组间无显著差异。卵子中抗体蛋白免疫组化定位结果表明, 未受精卵中IgM抗体集中分布在卵膜内侧边缘, 卵黄部分分布不明显。研究还发现, 卵巢液中抗体水平显著高于卵子匀浆液, 且免疫亲鱼组卵巢液中抗体水平显著高于对照组(P<0.05)。本实验验证了大菱鲆特异性抗体的母源传递性, 并揭示了在本免疫条件下的传递时效, 为进一步开展通过母源免疫对育苗早期疾病防控途径的探索奠定了基础。 |
| 关键词: 大菱鲆 母源免疫 抗体 亲鱼 卵子 |
| DOI：10.11693/hyhz201305026026 |
| 分类号: |
| 基金项目:国家自然科学基金项目资助, 31101888号, 2012.01—2012.12; 山东省自然科学基金项目资助, ZR2011CQ004号, 2011.07—2014.07; 国家鲆鲽类产业技术体系项目资助, CARS-50号, 2011.01—2015.01。 |
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| EFFECT OF MATERNAL IMMUNITY ON THE ANTIBODY IgM LEVEL IN TURBOT SCOPHTHALMUS MAXIMUS OFFSPRING |
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DING Fu-Hong, LEI Ji-Lin, HAN Ming-Ming, LIU Xin-Fu, MA Ai-Jun
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Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences
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| Abstract: |
| To understand the effect of bacterin on the antibody level in the offsprings of turbot Scophthalmus maximus, we vaccinated the broodstocks of the fish with Vibrio anguillarum bacterin, and studied the position of antibody in the turbot eggs. Results indicate that the antibody level in the eggs of vaccinated broodstock was significantly higher than that of the control (P<0.05). Within 48h post fertilization, the antibody level in embryos of vaccinated fish was significantly higher than un-vaccinated group (P>0.05), whereas, such difference was not detected after 48h. In addition, the maternal antibody was concentrated on the internal side of chorion, indicated by immunohistochemical analysis of egg samples. Therefore, the antibody could transfer through maternal immunity in turbot S. maximus, which may lay a foundation for future study on maternal immunity of turbot. |
| Key words: turbot Scophthalmus maximus maternal immunity antibody broodstock eggs |
