| 摘要: |
| 采用气升式光生物反应器培养系统, 进行了通气速率、氮浓度、Fe3+浓度、IAA浓度、CO2补充量等环境因子对蛋白核小球藻生长和油脂积累影响的研究。结果表明, 通气速率为2.0L/min时小球藻的生长速率和油脂产量最大分别为6.17mg/(L·d)和1.77mg/(L·d); 实验范围内氮浓度对小球藻油脂产量影响规律性不强; Fe3+浓度为0.1mmol/L时小球藻的生长速率最大为7.29mg/(L·d), Fe3+浓度为1.0mmol/L时小球藻油脂产量最高为2.54mg/(L·d); IAA浓度为1.0mg/L时小球藻的生长速率为7.47mg/(L·d), 油脂产量为2.89mg/(L·d), 二者都达到最大值; CO2补充量为6000mL/d时小球藻的生长速率最大为9.88mg/(L·d), 补充量为4000mL/d时小球藻油脂产量最高为3.86mg/(L·d)。经过条件优化, 小球藻油脂产量得到了显著的提高。 |
| 关键词: 蛋白核小球藻 光生物反应器 环境因子 生长速率 油脂积累 |
| DOI：10.11693/hyhz201305025025 |
| 分类号: |
| 基金项目:浙江省科技厅公益性研究项目, 2010C33066号; 浙江省创新团队项目, 2009R50012-6号; 宁波市科技攻关项目, 2010C10022号; 宁波市科技局国际合作项目, 2010D10012号。 |
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| EFFECTS OF ENVIRONMENTAL FACTORS ON GROWTH AND LIPID ACCUMULATION OF CHLORELLA PYRENOIDOSA IN PHOTOBIOREACTOR |
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CONG Feng, SUN Xue, XU Nian-Jun, CAI Xi-Li
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Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University,Marine Biotechnology Key Laboratory of Zhejiang Province
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| Abstract: |
| The effects of air aeration rate, nitrogen, Fe3+, IAA concentration, and CO2 supply volume on growth and lipid accumulation of microalgae Chlorella pyrenoidosa in airlift photobioreactor were studied. Results show that when the air aeration rate was 2.0L/min, the growth rate of C. pyrenoidosa was 6.17mg/(L·d) and the lipid production was 1.77mg/(L·d). During the experimental time, the nitrogen concentration did not affect the algal lipid production significantly. The optimal conditions of other factors were: Fe3+ concentration at 0.1mmol/L for the best growth rate [7.29mg/(L·d)], and 1.0mmol/L for best lipid production [2.54mg/(L·d)]; IAA concentration at 1.0mg/L for both the growth rate and lipid production [7.47 and 2.89mg/(L·d), respectively]; CO2 supply volume at 6000mL/d for the growth rate [9.88mg/(L·d)], and at 4000mL/d for the lipid production [3.86mg/(L·d)]. In this experiment, the lipid production in the optimal conditions is significantly higher than the control group. |
| Key words: Chlorella pyrenoidosa photobioreactor environmental factors growth rate lipid accumulation |
