| 摘要: |
| 以来源于泰国的菌株TG06003 作对照, 对采自海南地区的27 株溶藻弧菌(Vibrio alginolyticus)环境菌株进行了随机扩增多态性DNA 标记(RAPD)分析, 其中6 株为毒力菌株, 另外22 株为非毒力菌株。实验共得到70 个多态性条带, 多态性比率(PPB)为98.6%。基因多样度的算术平均数和加权平均数分别为0.922 和0.454 4, Shannon’s 信息指数的算术平均数和加权平均数分别为0.139 1 和0.171 3。遗传相似度为0.442 9～0.885 7, 遗传距离为0.121 4～0.752 0。将溶藻弧菌分为毒力菌株和非毒力菌株两个类群进行分析, 结果表明, 毒力菌株的遗传多样性丰富, 它们的基因多样度和Shannon’s 信息指数明显高于非毒力菌株; 毒力菌株HN08811、HN08155、HN08809、HN08813 明显聚为一类, 另外2 株毒力菌株分别与非毒力菌株HN08304 和HN08803 聚为一类, 溶藻弧菌毒力菌株存在多种遗传型。 |
| 关键词: 溶藻弧菌(Vibrio alginolyticus) RAPD 毒力菌株 遗传多样性 |
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| 基金项目:国家科技支撑计划项目(2009BAB44B02); 国家自然科学基金项目(30760190, 31060360); 霍英东教育基金会高等院校青年教师基金基础性研究课题(121030); 农业科技成果转化项目(2009GB2E200302) |
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| RAPD analysis of genetic diversity of Vibrio alginolyticus isolated from Hainan province |
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| Abstract: |
| Taking the Thai strain TG06003 as a control, 27 strains of Vibrio alginolyticus collected from Hainan maricultural environment were analyzed using the random amplified polymorphic DNA marker (RAPD) method. Six strains were virulent and the other 22 strains were non-virulent. Seventy polymorphic bands were produced by PCR with eight selected primers, and the PPB was 98.6%. The arithmetic average and the weighted average of gene diversity were 0.922 and 0.454 4, respectively, and the arithmetic average and the weighted average of Shannon’s information index were 0.1391 and 0.171 3, respectively. Distribution of genetic similarity was between 0.442 9～0.885 7, and genetic distances were distributed between the 0.121 4～0.752 0. Virulent strains HN08811, HN08155, HN08809, and HN08813 were clearly clustered together. However, the other two virulent strains were clustered with non-virulent strains. The gene diversity of virulent strains was high, and their genetic diversity and Shannon’s information index were significantly higher than those of non-virulent strains. Our results indicate that virulent strains and non-virulent strains of Vibrio alginolyticus have multiple genetic types. |
| Key words: Vibrio alginolyticus RAPD, virulent strains genetic diversity |
