| 摘要: |
| 对中国威海近海海域的紫贻贝、牡蛎、花蛤、毛蛤、扇贝、海兔等6 种海洋贝类的附生细菌进行了分离培养, 筛选得到100 株细菌。从中选出45 株有代表性的菌株, 克隆其16S rDNA 序列, 进行分子鉴定。结果表明, 这些细菌分布在Arthrobacter, Bacillus, Bizionia, Brevundimonas, Cellulophaga,Cobetia, Gelidibacter, Kocuria, Krokinobacter, Lacinutrix, Marinobacter, Microbacterium, Micrococcus,Paracoccus, Planomicrobium, Pseudoalteromonas, Psychrobacter, Salinibacterium, Shewanella, Tenacibaculum和Vibrio 共21 个属中。利用平板点种法对这100 株菌株进行了抗菌活性筛选, 结果显示有2株细菌具有明显的抗菌活性。 |
| 关键词: 细菌多样性 海洋贝类 16S rDNA 区系分析 拮抗 |
| DOI： |
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| 基金项目:山东大学威海分校科研立项资助项目(A10021) |
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| Isolation and biodiversity analysis of bacteria attached on the marine shellfish and selection of vibrios-antagnism bacteria |
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| Abstract: |
| A preliminary screening of bacteria attached on the marine shellfish from six marine shellfishes(Mytilus edulis, Concha ostreae, Ruditapes variegate, Anadara uropygimelana, Placopecta magellanicus, and Ovula ovum) in the coastal waters of Weihai yielded 100 isolates, among which 45 strains were selected for further analysis. To investigate the phylogenetic position of these strains, the 16S rDNA sequences were cloned, sequenced, and compared with those of related strains and a molecular phylogenetic dendrogram was constructed based on genetic distance analysis．The strains were classified as members of genera Arthrobacter,Bacillus, Bizionia, Brevundimonas, Cellulophaga, Cobetia, Gelidibacter, Kocuria Krokinobacter, Lacinutrix, Marinobacter, Microbacterium, Micrococcus, Paracoccus, Planomicrobium, Pseudoalteromonas ,Psychrobacter, Salinibacterium, Shewanella, Tenacibaculum, and Vibrio. The antimicrobial activity of the 100 strains were screened by the agar flat seeding method. The experiment results showed that 2 strains had obvious antimicrobial activity. |
| Key words: bacterial diversity marine shellfish 16S rDNA flora analysis antagonistic |
