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铁促进海水小球藻油脂积累的动态过程
刘志媛1,2, 王广策1
1.中国科学院海洋研究所;2.中国科学院研究生院
摘要:
许多微藻被认为是制备生物油燃料的理想原料,培养液中高浓度的Fe3+对海水小球藻(Chlorella vulgaris) 的油脂积累有明显的促进作用。采用荧光分光光度法对尼罗红染色后的海水小球藻的油脂积累过程进行了观测,同时测定了铁对小球藻生长和叶绿素含量的影响。结果表明,高铁处理的小球藻出现了两次油脂积累高峰,且第2次脂积累的量显著高于第1次。
关键词:  脂  微藻  FeCl3  叶绿素  荧光分光光度法
DOI：
分类号:
基金项目:国家863计划项目( 2006AA05Z112, 2007AA09Z110,2007AA09Z406)
Dynamics of lipid accumulation in marine microalga Chlorella vulgaris promoted by iron
Abstract:
Some microalgae are proposed as good candidates for biodiesel production. Lipid accumulation in a marine microalga Chlorella vulgaris can be promoted by high level of Fe3+ concent ration in the media. The dynamics of lipids accumulation promoted by iron in Nile red-stained C. vulgaris was traced with spectrofluorimetry method. The effect of iron on the growth and chlorophyll content of C. vulgaris was also measured. The results showed that notable lipid accumulation occurred twice in cells under high iron condition and much more lipid accumulated in the second period.
Key words:  lipid  microalga  FeCl3  chlorophyll  spectrofluorimet ry
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