| 摘要: |
| 从采自大连近海的红藻鸭毛藻(Symphyocladia latiuscula) 中分离到一株肉座菌目真菌(Hypocreales sp.) ,对其发酵代谢产物的化学成分进行了研究。利用正相硅胶柱层析、葡聚糖凝胶Sephadex LH-20 柱层析、制备薄层层析(PTLC)以及重结晶等分离手段,并通过一维、二维核磁共振技术、质谱技术等从该菌发酵液中分离鉴定了10个化合物:双酚A(1);邻羟基苯甲酸(2);吲哚甲酸(3);吲哚乙酸(4);N-乙酰色胺(5);(22E,24R)-麦角甾-7,9,22-三烯-3β-醇(6);过氧化麦角甾醇(7); (22E,24R)-5α,6α-环氧麦角甾-8,22-二烯-3β,7α-二醇(8);(22E,24R)-麦角甾-7,22-二烯-6β-甲氧基-3β,5α-二醇(9);啤酒甾醇(10)。这些化合物均为首次从该菌中分离得到,其中化合物1为首次作为天然产物分离得到。 |
| 关键词: 鸭毛藻(Symphyocladia latiuscula) 内生真菌 肉座菌 化学成分 |
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| 基金项目:国家自然科学基金项目(30530080);山东省科技发展计划项目(2006GG2205023);海洋药物教育部重点实验室开放基金项目[KLMD(OUC)2004] |
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| Chemical constituents of Hypocreales sp., an endophytic fungus derived from the red alga Symphyocladia latiuscula |
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| Abstract: |
| From the fermentation broth of Hypocreales sp. , an endophytic fungus derived from the red alga S ym p hyocl adi a l at i uscul a , ten compounds were isolated by the normal phase silica gel, Sephadex LH-20 column chromatography separation, and by the preparative thin layer chromatography( PTLC) as well as by re-crystallization. Structures of purified compounds were elucidated by analysis of spectroscopic data including MS, 1D and 2D NMR as bisphenol A(1), salicylic acid(2), 3-indoleformic acid(3), 3-indoleacetic acid(4), N-acetyltryptamine(5), 22E,24R-ergosta-7,9 ,22-trien-3β-ol(6), ergosterol peroxide(7), and 22E,24R-5α, 6α-epoxyergosta-8, 22-dien-3β, 7α-diol(8), ergosta-7, 22-dien-6β-methoxy-3β, 5α-diol(9), cerevisterol(10). All the compounds were obtained from this fungus for the first time and compound(1) was reported as a new natural product. |
| Key words: Symphyocladia latiuscula endophytic fungus Hypocreales sp. chemical constituents |
