| 摘要: |
| 利用甲壳素作为唯一碳源从土壤中筛得的真菌d,与细菌s混合发酵,对其最适产酶条件、所产甲壳素粗酶的制取以及粗酶性质进行初步研究。结果表明,混合发酵产酶的能力与单独发酵相比显著提高,混合发酵最适培养基组分为(×10-2 g/mL):尿素1.0,K2HPO4 0.07,KH2PO4 0.03,MgSO4 0.05,酵母粉0.5,甲壳素1.0,pH 5.5,接种量2%（体积分数）,32℃,130 r/min培养72 h,500 mL锥形瓶培养基装量为100 mL。经过优化,发酵液中甲壳素酶活力从0.73 U/mL提升到1.19 U/mL。通过对粗酶性质初步的研究,发现该酶有较好的稳定性。 |
| 关键词: 甲壳素酶 酶活力 培养条件优化 酶学性质 |
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| Optimization of the mixed fermentation conditions for chitinase production and some properties of the crude enzyme |
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| Abstract: |
| As an original chitinase-producing strains and straind, screening from the soil, the study shows the mixed fermentation we get is a higher chitinase-producing. The optimal compositions of the medium for the strain producing chitinase are as follows(×10-2 g/mL): CO（NH2）2 1.0,K2HPO4 0.07,KH2PO4 0.03, MgSO4 7H2O 0.05,yeast extract 0.5 and the chitin 1.0 （pH 5.5）.The optimal cultivation conditions for the strain producing chitinase were 500 mL flask containing 100mL medium, 2% amount of inoculation, 32℃incubating temperature and shaking cultivation at 130 r/min for 72hours. The most activity of chitinase of fermentation broth is 1.19 U/mL.Acorrding to the study, the crude enzyme showed a good stability. |
| Key words: chitinase activity optimization of fermentation condition enzyme characterization |
