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一株深海嗜低温萘降解细菌Nah-1的分离及降解基因研究
王新新1,2, 党宏月2, 王春艳,2, 唐学玺1
1.中国海洋大学海洋生命学院;2.中国科学院海洋研究所海洋地质与环境重点实验室
摘要:
以萘为唯一碳源和能源从南冲绳海槽深海沉积物中分离得到一株能降解萘的海洋噜低温细菌Nah-1,测定了该菌的最适生长条件及生长曲线。16S rRNA基因(16S rDNA)序列同源性分析表明该菌属于解环菌属(Cycloclasticus)。PCR扩增萘降解基因得到目标片段,比对结果表明,相似度最高的基因phnA1来自Cycloclasticus sp.A5,为99%,该基因编码的蛋白是萘双加氧酶大亚基。
关键词:  萘  多环芳烃  生物降解  双加氧酶  深海嗜低温细菌
DOI：
分类号:
基金项目:中国科学院知识创新工程重要方向项目(KZC)(2-YW-211-03)
Islation of a PAHs degrading marine bacterium Nah-1 and its degradative gene
Abstract:
A PAHs-degrading psychrophilic bacterium,with naphthalene as sole carbon and energy source, was isolated from deep-sea sediment of Southern Okinawa Trough. The growth curve of this bacterium was determined and the optimum growth conditions of this bacterium were studied. 16S rDNA sequences analysis indicated that it belongs to the genus Cycloclasticus. A fragment of the degrading dioxygenase gene was obtained through PCR from Nab-1. And sequence alignment revealed that it was similar to the phnA1 gene of Cycloclasticus sp. AS, which encoded a putative naphthalene dioxygenase large subunit.
Key words:  naphthalene  polycyclic aromatic hydrocarbon  biodegradation  dioxygenase  psychrophilic deep-sea bacterium
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