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南海北部陆坡深水区沉积物细菌多样性调查
王 鹏1, 李 涛1
同济大学海洋地质国家重点实验室
摘要:
采用分子生物学手段,调查南海北部陆坡深水区沉积物细菌多样性。分析表明其细菌分成5个类群,变形细菌门(Proteobacteria),厚壁菌门(Firmicutes),浮霉菌门(Planctomycetes),拟杆菌门(Cytophaga/Flexibacteria/Bacteroides,简称CFB)及脱铁杆菌门(Deferribacteres)。分别占总体的34%,38%,18%,4%,6%。其中60%以上的克隆子与硫代谢相关,20%克隆子最相近序列来自于油污染环境,说明硫代谢是该区域物质代谢的重要组成,同时该区域有油气渗透的烃类物质存在并影响着该区域的微生物群落结构。
关键词:  南海北部陆坡  细菌多样性  微生物勘探
DOI:
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基金项目:国家重点基础研究发展规划资助项目(2007CB815904) ;国家自然科学基金资助项目(40606032) ;国家“十一五”大洋专项基金资助项目(DYXM-115-02-2-17) ;同济大学青年优秀人才培养行动计划资助项目(2006KJ056)
Phylogenetic analysis of bacterial community in deep-sea sediment from northern slope of the South China Sea
Abstract:
The bacterial community in one deep-sea sediment sample from northern slope of the South China Sea was investigated by molecular methods. Aphylogenetic analysis revealed that the bacterial population was composed of 5 major lineages of the domain bacteria, Proteobacteria (34%), Firmicutes(38%), Planctomycetes(18%), Cytopahga-Flexibacteria-Bacteroides(CFB)(4%) and Deferribacteres(6%). Over 60% clones were related with metabolism of sulfur, indicating the important role of sulfur cycle in this area. 20% clones show high similarity with clones from an environment impacted by gasoline, indicating the existence of hydrocarbon from petroleum resources.
Key words:  northern slope of the South China Sea  bacterial diversity  microbial prospecting
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