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海鞘内生真菌焦曲霉Aspergillus ustus TK-5的化学成分研究
李 莉1,2, 李晓明1, 李洪雷1, Belma Konuklugil3, 李 鑫1, 王斌贵1
1.中国科学院 海洋研究所 实验海洋生物学重点实验室;2.中国科学院大学;3.Department of Pharmacognosy, Faculty of Pharmacy, Ankara University
摘要:
焦曲霉(Aspergillus ustus)TK-5是分离自土耳其海域海鞘(Pyura momus)新鲜组织中的一株内生真菌, 利用正相与反相硅胶柱层析、葡聚糖凝胶Sephadex LH-20柱层析以及高效液相制备等色谱方法从其发酵产物中分离得到17 个化合物, 通过一维、二维核磁共振、质谱等技术鉴定了所有化合物的结构, 分别为血苋烷型倍半萜类化合物 strobilactone A(1), ustusolate E(2), ustusolate C(3), ustusolate D(4), 11-hydroustusolate E(5), 11, 6’-hydroustusolate E(6), (2’E, 4’E, 6’E)-6-(1’-carboxyocta-2’, 4’, 6’-triene)-11, 12-epoxy-9, 11-dihydroxydrim-7-ene(7), 12-hydroxy-6-epi-albrassitriol (8), ustusolate A (9), deoxyuvidin B (10)和二倍半萜类化合物 6-epi-ophiobolin G(11), (6α)-21, 21-O-dihydroophiobolin G(12), 6-epi-ophiobolin K(13), ophiobolin P(14) ophiobolin H(15), ophiobolin Q(16)及ophiobolin R(17)。活性筛选表明化合物2、6、7、9、11 和13对神经氨酸酶具有一定的抑制活性, 其半数抑制浓度(IC50) 分别为31.8、37.3、28.4、36.8、46.6和37.6 μmol/L。
关键词:  海鞘  内生真菌  焦曲霉  萜类  神经氨酸酶抑制活性
DOI:10.11759/hykx20180320002
分类号:
基金项目:国家自然科学基金委员会-山东省海洋科学研究中心联合基金(U1606403)
Chemical constituents of Aspergillus ustus TK-5, an endophytic fungus derived from the ascidian Herdmania momus
LI Li,LI Xiao-ming,LI Hong-lei,BELMA Konuklugil,LI Xin,WANG Bin-gui
Abstract:
Cultivation of the fungal strain Aspergillus ustus TK-5, an endophytic fungus which was isolated from the fresh tissue of ascidian Herdmania momus, resulted in the identification of 17 compounds. These compounds were isolated by a combination of silica gel, Sephadex LH-20, and Lobar LiChroprep RP-18 column chromatography as well as by preparative high-performance liquid chromatography (pHPLC). The structures were elucidated to be drimane sesquiterpenoids (1–10) and sesterterpenes (11–17) by analysis of their spectroscopic data, including strobilactone A (1), ustusolate E (2), ustusolate C (3), ustusolate D (4), 11-hydroustusolate E (5), 11, 6’- hydroustusolate E (6), (2’E, 4’E, 6’E)-6-(1’-carboxyocta-2’, 4’, 6’-triene)-11, 12-epoxy-9, 11-dihydroxydrim-7-ene (7), 12-hydroxy-6-epi-albrassitriol (8), ustusolate A (9), deoxyuvidin B (10), 6-epi-ophiobolin G (11), (6α)-21, 21-O-dihydroophiobolin G (12), 6-epi-ophiobolin K (13), ophiobolin P (14), ophiobolin H (15), ophiobolin Q (16), and ophiobolin R (17). Regarding to the neuraminidase inhibitory activity, compounds 2, 6, 7, 9, 11, and 13 were found to possess moderate activity, with IC50 values of 31.8, 37.3, 28.4, 36.8, 46.6, and 37.6 μmol/L.
Key words:  Ascidians  Endophytic fungus  Aspergillus ustus  Terpenoids  Neuraminidase inhibitory activity
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