| 摘要: |
| 首次在长牡蛎(Crassostrea gigas)中克隆得到一种新的贝壳基质蛋白nacrein-like protein F3 的全长cDNA 序列。nacrein-like protein F3 基因cDNA 全长1499bp, 其中编码区长度为1242bp, 编码一条含413 个氨基酸残基的多肽链。氨基酸序列比对和结构域分析均表明其为合浦珠母贝(Pinctada fucata)nacrein 的同源蛋白, 含有1 个保守的α-碳酸酐酶结构域, 但由于重复结构域的插入, α-碳酸酐酶结构域被间隔成2 个亚结构域。系统进化分析显示nacrein-like protein F3 与贻贝(Mytilus californianus)nacrein-like protein 进化关系最近。此外, 在软体动物中, 双壳纲nacrein-like proteins 进化速度相对较快, 推测与寒武纪时期剧烈的环境变化有关, 如影响贝壳形成的海水化学变化。 |
| 关键词: 长牡蛎(Crassostrea gigas) nacrein-like protein 碳酸酐酶结构域 系统进化分析 |
| DOI：10.11759/hykx20130331002 |
| 分类号: |
| 基金项目:国家“973”计划资助项目(2010CB126401); 国家自然科学基金项目(40730845); 现代农业产业技术体系构建研究专项基金项目;山东省泰山学者计划及山东泰山学者攀登计划项目 |
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| Molecular cloning and characterization of nacrein gene in Pacific oyster (Crassostrea gigas) |
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| Abstract: |
| The full length cDNA sequence of nacrein-like protein F3 was cloned from Pacific oyster (Crassostrea gigas) for the first time. Nacrein-like protein F3 cDNA has 1499 bp, containing a 1242 bp CDS that encod a peptide of 413 amino acids. Multiple alignment and conserved domain analysis showed that this peptide is a homolog of nacrein (Pinctada fucata), containing a carbonic anhydrase (CA) domain, which is flanked by inserted repeat sequences. Phylogenetic analysis indicated that nacrein-like protein F3 is more closely related to nacrein-like protein in Mytilus californianus than other nacrein-like proteins. Moreover, the nacrein-like proteins in bivalves seem to evolve relatively fast, which is presumably caused by the fast changing Cambrian environment, especially the marine chemical composition, which can affect shell biomineralization. |
| Key words: Pacific oyster (Crassostrea gigas) nacrein-like protein carbonic anhydrase domain phylogenetic analysis |
