引用本文:	孙颖颖,王辉.球等鞭金藻胞外多糖的体外抗氧化活性和理化性质的初步分析[J].海洋科学,2013,37(5):45-49.

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球等鞭金藻胞外多糖的体外抗氧化活性和理化性质的初步分析
孙颖颖¹, 王辉²
1.淮海工学院江苏省海洋生物技术重点实验室;2.北京市医疗器械检验所

摘要:

前期研究采用离子交换柱层析和凝胶柱层析, 制备到一种球等鞭金藻胞外纯多糖ECPSⅢ。在此基础上, 采用化学比色法研究了ECPSⅢ的体外抗氧化活性; 同时, 通过测定ECPSⅢ中的硫酸基和糖醛酸含量, 初步分析了ECPSⅢ的理化性质。结果表明, ECPSⅢ具有清除超氧阴离子(O₂·^-)、羟基自由基(·OH)和过氧化氢(H₂O₂)等活性氧的能力和一定的还原力。其中, ECPSⅢ对O₂·^-和·OH 的清除能力较强。ECPSⅢ中的硫酸基和糖醛酸含量分别为76.90 mg/g 和17.1%, 是一种富含硫酸基和糖醛酸的酸性多糖。

关键词: 球等鞭金藻胞外多糖抗氧化活性理化性质

DOI：

分类号:

基金项目:连云港市科技攻关工业项目(CG0803-1); 淮海工学院人才引进科研启动项目(KQ08001)

Study of in vitro antioxidation and physical and chemical characteristics analysis of extracellular polysaccharides isolated from Isochrysis galbana

Abstract:

The extracellular polysaccharides, ECPSⅢ, has been successfully isolated from marine microalgae Isochrysis galbana through a combination of anion-exchange column chromatography and repeated gel chromatography in previous study. Its in vitro antioxidant activities and physical and chemical properties were investigated. The results showed that ECPSⅢ has good antioxidant activities and perticularly it has relative stronger scavenging activities against superoxide radical and hydroxyl radical. ECPSⅢ is a acidic polysaccharide rich in sulfuric acid radicals and uronic acid. The contents of sulfuric acid radicals and uronic acid were 76.90 mg/g and 17.1% in ECPS Ⅲ, respectively.

Key words: Isochrysis galbana extracellular polysaccharides antioxidation activity physical and chemical characteristics