| 摘要: |
| 将羊栖菜(Sargassum fusiforme)经浸提、萃取和超滤等步骤获得不同分子质量的褐藻多酚。用普鲁士蓝法检测其总抗氧化能力; 采用DPPH、羟自由基试剂盒、超氧阴离子试剂盒评价其对DPPH、羟自由基和超氧阴离子的清除能力。结果显示, 羊栖菜褐藻多酚主要以高分子质量的形式存在; 乙酸乙酯相组分各项抗氧化指标均好于水相, 且乙酸乙酯相分子质量大于30 000 组分对羟自由基与超氧阴离子的清除能力比没食子酸、Vc和VE强。 |
| 关键词: 羊栖菜(Sargassum fusiforme) 不同分子质量褐藻多酚 抗氧化活性 |
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| 基金项目:广东省科技计划项目(2010B030800016); 湛江市科技计划项目(2012E02); 广东医学院科技创新团队基金(STIF201104) |
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| Antioxidative Activities of phlorotannins with different molecular weight extracted from Sargassum fusiforme |
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| Abstract: |
| The antioxidative activities of phlorotannins with different molecular weight extracted from Sargassum fusiforme were investigated. The prussian blue method was used to detect their total oxidant capacity, and DPPH, hydroxyl free radical kit and superoxide anion kit were used to measure their scavenging ability, respectively. The results showed that S. fusiforme mainly contains high molecular phlorotannins. Components from ethyl acetate layer exhibited stronger antioxidant capacities than those from water layer. The component with molecular weight more than 30000 from ethyl acetate layer exhibited stronger hydroxyl free radicals and superoxide anion scavenging capabilitythan gallic acid, Vc and VE. |
| Key words: Sargassum fusiforme phlorotannins with different molecular weight antioxidative activity |
