引用本文:	李超柱,陈艳华,陈艳辉,张艳秋,方怀义.牡蛎活性肽的分离及其免疫抑制作用的实验研究[J].海洋科学,2013,37(4):52-56.

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牡蛎活性肽的分离及其免疫抑制作用的实验研究
李超柱¹, 陈艳华², 陈艳辉¹, 张艳秋¹, 方怀义¹
1.钦州学院;2.广西医科大学附属肿瘤医院

摘要:

采用动物蛋白酶对牡蛎蛋白质进行酶解, 获得牡蛎活性肽, 将分子质量小于3 kD 的活性肽分别采用MTT 法、体外抑制小鼠脾淋巴细胞增殖试验和小鼠体外脾混合淋巴细胞增殖活性实验, 并通过ELISA 法检测牡蛎活性肽作用后小鼠脾脏淋巴细胞上清中分泌的细胞因子IL-2、IFN-γ的变化。试验结果表明, 牡蛎活性肽对小鼠脾淋巴细胞增殖具有一定的免疫抑制作用, 经牡蛎活性肽作用后的小鼠脾脏淋巴细胞上清中细胞因子IL-2、IFN-γ分泌减少。

关键词: 牡蛎活性肽免疫抑制作用淋巴细胞增殖

DOI：

分类号:

基金项目:广西自然科学基金资助(2010GXNSFA013062); 广西教育厅面上项目资助(200911MS241)

Separation and immunosuppressive activity analysis of the bioactive peptides from oyster

Abstract:

The bioactive peptides of oyster were obtained by animal protease enzyme from oyster protein. The bioactive peptides molecular weight less than 3kD were analyzed using MTT assay, in vitro mice spleen lymphocytes proliferation assay and in vitro mixed lymphocyte proliferation assay. The changes of secretory cytokines IL-2 and IFN-γ in supernatant of mouse splenic lymphocyte after bioactive peptides treatment were analyzed using ELISA assay． The results showed that Oyster peptide can suppress the proliferation of spleen lymphocytes in mice and reduce the secretory cytokines IL-2 and IFN-γ in supernatant of mouse splenic lymphocyte.

Key words: Oyster bioactive peptides immunosuppressive effects lymphocyte proliferation