引用本文:	朱本伟,李富超,秦松.海洋链霉菌M506 的鉴定及代谢产物抑菌活性初步研究[J].海洋科学,2012,36(12):45-49.

【打印本页】【HTML】【下载PDF全文】【查看/发表评论】【下载PDF阅读器】【关闭】

过刊浏览高级检索

本文已被：浏览 2367次下载 3476次	码上扫一扫！
分享到：微信更多字体:加大+\|默认\|缩小-
海洋链霉菌M506 的鉴定及代谢产物抑菌活性初步研究
朱本伟^1,2,3, 李富超², 秦松¹
1.中国科学院烟台海岸带研究所;2.中国科学院海洋研究所海洋生物学重点实验室;3.中国科学院研究生院

摘要:

自胶州湾沉积物中分离出海洋链霉菌M506, 根据其形态特征观察、生理生化特征试验及基于16S rDNA 序列系统进化分析, 初步鉴定该菌为灰略红链霉菌。采用不同培养条件对其进行小规模发酵,利用金黄色葡萄球菌(Staphylococcus aureus), 大肠杆菌(Escherichia coli), 枯草芽孢杆菌(Bacillus subtilis)和苏云金芽孢杆菌(Bacillus thuringiensis)四株细菌作为指示菌, 检测其抑菌活性。结果表明, 该菌株代谢产物粗提物对于金黄色葡萄球菌、枯草芽孢杆菌和大肠杆菌等具有显著抑制活性。

关键词: 海洋链霉菌抑菌活性鉴定

DOI：

分类号:

基金项目:国家自然科学基金项目(31000037); 中国科学院知识创新工程项目(KZCX2-YW-JC201); 山东省自然科学基金项目(JQ200914,ZR2009EQ004); 南通市科技项目(AS2011012)

Identification of marine Streptomyces sp. M506 and the antimicrobial activity of its metabolites

Abstract:

Marine Streptomyces sp. M506 was isolated from the sediment of Jiaozhou Bay. According to morphological observations, physiological biochemical characteristics and 16S rDNA phylogenetic analysis, the strain M506 was identified to be Streptomyces griseorubens. In this study, the culture conditions had been optimized and six kinds of medium were used to culture the strain M506. The crude extracts from M₂+ and SM medium exhibited distinct antimicrobial activities against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Bacillus thuringiensis. The result indicated that the culture condition optimization is an improved strategy to screen bioactive metabolites from marine Streptomyces.

Key words: Marine Streptomyces antimicrobial activity identification