| 引用本文: | 隋 娟,徐世宏,王文琪,马道远,肖志忠,肖永双,刘清华,李 军.牙鲆与夏鲆杂交及回交子代胚胎发育及早期生长比较[J].海洋科学,2012,36(6):54-58. |
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| 摘要: |
| 通过人工授精技术对牙鲆(Paralichthys olivaceus )和夏鲆( Paralichthys dentatus )进行了正反交及回交实验, 并比较了正反交、回交子代的胚胎发育时序和仔稚幼鱼的生长。结果表明: 孵化水温18.0±0.5℃下, 初孵仔鱼破膜用时为: 牙鲆41 h, 正交鲆47 h, 回交牙鲆42 h30 min, 回交夏鲆44 h;16.5±0.5℃下, 夏鲆61 h 40 min, 反交鲆66 h。正交及两组回交子代在胚胎期均可正常发育。反交子代卵裂期出现异常, 仅少数胚胎可以孵化。胚胎孵化后, 在1～64 日的培养过程中, 反交鲆于3～4 d 内全部死亡, 回交牙鲆发育至18 日龄时全部死亡。正交鲆和回交夏鲆可正常发育, 与牙鲆没有明显差别。 |
| 关键词: 牙鲆(Paralichthys olivaceus) 夏鲆(Paralichthys dentatus) 杂交 回交 早期发育 |
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| 基金项目:现代农业产业技术体系建设专项资助项目(nycytx-50); 国家农业成果转化资金(2009GB24910539); 中国科学院知识创新项目(KSCX2-EW-B-3); 山东省科技发展计划项目(2011GHy11530); 山东省中青年科学家科研奖励基金(BS2009HZ007); 青岛市基础研究计划项目(09-1-3 -11-jch) |
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| Comparison of embryonic development and growth of hybrids and backcrosses from Paralichthys olivaceus and Paralichthys dentatus |
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| Abstract: |
| Reciprocal and back crosses were carried out on Paralichthys olivaceus (Po) and Paralichthys dentatus (Pd) by artificial fertilization. The embryonic and larvae development were compared among the six groups. The results showed that, at 18.0℃±0.5℃, the embryos of P. olivaceus, Po(♀) × Pd(♂), F1(♀) × Po(♂) and F1(♀) ×Pd(♂) took 41, 47, 42.5, and 44 h to hatch, respectively. At 16.5℃±0.5℃, the embryos of P. dentatus and Pd (♀) ×Po (♂) took 61 h 40 min and 66 h to hatch, respectively. For Po(♀) × Pd(♂) and two back crosses, the embryos developed normally. While most of the embryos from Pd (♀) ×Po (♂) developed with abnormal cleveage, only few embryos hatched out normally. During a 64-day cultivation of the post hatching larvae, all hybrids of Pd(♀) ×Po (♂) died at 3-4 days post hatching (dph) and F1(♀) × Po(♂) offspring died completely at 18 dph. The progenies of Po(♀) × Pd(♂) and F1(♀) × Pd(♂) experienced normal development and growth stages as that of P. olivaceus. |
| Key words: Paralichthys olivaceus Paralichthys dentatus hybridization backcross early development |
