| 摘要: |
| 采用放射性免疫方法测定和比较了人工诱导的雌核发育牙鲆(Paralichthys olivaceus)与对照组牙鲆血清中睾酮(T)和雌二醇(E2)水平。研究发现, 雌核发育牙鲆个体中的T 含量显著高于对照组牙鲆个体(P<0.05), 而其E2 含量则略低于对照组个体; 在雌核发育牙鲆中, 卵巢发育不正常的个体比发育正常的个体中的T 含量稍高, 而E2 含量则显著低于正常雌核发育牙鲆个体和对照组牙鲆个体(P< 0.05)。可以推测, 雌核发育牙鲆和对照组牙鲆体内T 和E2 水平存在差异, 且雌核发育牙鲆中卵巢发育正常与不正常的个体之间T 和E2 也存在差异, 而这种差异可能导致了雌核发育牙鲆部分个体卵巢发育异常。本研究将为正常二倍体及雌核发育牙鲆性腺发育和内分泌学研究提供信息支持。 |
| 关键词: 牙鲆(Paralichthys olivaceus) 雌核发育 卵巢 T E2 |
| DOI： |
| 分类号: |
| 基金项目:中央级公益性科研院所基本科研业务费项目(东2009M05);公益性行业(农业)科研专项经费项目(200903046); 国家“863”计划资助项目(2006AA10A404); 鲆鲽类产业技术体系(nycytx-50); 山东省自然科学基金资助项目(ZR2010CM007) |
|
| Comparison of the levels of T and E2 in normal and gynogenetic Paralichthys olivaceus |
|
|
| Abstract: |
| Levels of serum T and E2 were analyzed by radioimmunoassay (RIA) in gynogenetic and control diploid individuals of Paralichthys olivaceus. Gynogenetic individuals had higher T levels and lower E2 levels than those control diploid individuals. In addition, higher T level and lower E2 level are observed in individuals with abnormal ovary when compared with gynogenetic and control diploid flounders. We suggested that there might be difference in serum T and E2 levels between gynogenetic and control diploid P. olivaceus, and also between gynogenetic individuals with normal and abnormal ovary development. These differences probably resulted in the abnormal ovary development of some gynogenetic individuals. The results will provide information to study ovary development and endocrinology of normal and gynogenetic flounders. |
| Key words: Paralichthys olivaceus gynogenesis ovary T E2 |
