| 摘要: |
| 采用生物化学方法研究了长牡蛎(Crassostrea gigas) 早期胚胎发育过程中脂肪酸、氨基酸组成变化情况, 分别获取受精后0、6、12、24 h 的胚胎或幼虫进行检测和分析, 结果如下: 在受精卵中共检测到27 种脂肪酸, 豆蔻酸(14:0)、软脂酸(16:0)、硬脂酸(18:0), EPA(20:5ω3)和DHA(22:6ω3)占总脂肪酸含量在各时期均较高; 随着发育进行, 饱和脂肪酸和单不饱和脂肪酸占总脂肪酸的比例不断增大,而多不饱和脂肪酸所占比例逐渐下降, ω3 系列和ω6 系列脂肪酸比例有不同程度的下降。共检测到16种氨基酸, 包括7 种必需氨基酸, 7 种非必需氨基酸和2 种半必需氨基酸, 总氨基酸含量随着发育进行不断降低。 |
| 关键词: 长牡蛎 胚胎发育 脂肪酸 氨基酸 |
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| 基金项目:国家863计划项目(2006AA10A401); 国家自然科学基金项目(30471326); 山东省博士基金(2005BS07002) |
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| Changes in fatty acid and amino acid composition during early development of the Pacific oyster (Crassostrea gigas) |
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| Abstract: |
| The compositions and contents of fatty acids and amino acids during the early embryonic development of the Pacific oyster (Crassostrea gigas) were analyzed by biochemical methods. Embryos or larvae sampled at 0, 6, 12, and 24 h post-fertilization (PF) were taken and measured. There were 27 fatty acids detected in the zygotes (0 h post-fertilization) and the proportions of myristic acid (14:0), palmitic acid (16:0), stearic acid (18:0), EPA (20:5ω3)and DHA(22:6ω3) were relatively high during 0~24 h PF; the proportions of the saturated fatty acids and also the monounsaturated fatty acids to the total fatty acids increased with the embryonic development while the poly-unsaturated fatty acids decreased gradually. Sixteen amino acids were detected, including seven essential amino acids, seven non-essential amino acids, two semi-essential amino acids; the total amino acids decreased with development. |
| Key words: Crassostrea gigas embryonic development fatty acid amino acid |
