| 摘要: |
| 厦门海水网箱养殖石斑鱼春、夏季节暴发性疾病经过流行病学调查和病原检测,确认主要病原为病毒性神经坏死病毒,病鱼外观表现典型的神经异常症状。应用逆转录聚合酶链式反应(RT-PCR)技术检测5种石斑鱼:赤点石斑鱼(Epinephelus akaara)、青石斑鱼(E.awoara)、云纹石斑鱼(E.moara)、紫石斑鱼(E.lanceolatus)、马拉巴石斑鱼(E.malabaricus)。检测76条石斑鱼,检出率90%;病鱼的脑、视网膜 |
| 关键词: 石斑鱼属(Epinephelus) 神经坏死病毒 逆转录聚合酶链式反应 |
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| Epidemiologic investigation of viral nervous necrosis in cage-cultured rockfishes in Xiamen |
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| Abstract: |
| A serious epidemic disease usually breaks out in spring and summer in cage-cultured rockfishes in Xiamen,Fujian province,China,and infected fishes appear abnormal in the nervous system.After epidemiologic investigation and pathogenic assay,the nervous necrosis virus (NNV) was identified to be the main pathogen. Seventy-six rockfishes,beloging to five kinds: Epinephelus.akaara,E.awoara,E.moara,E.lanceolatus and E. malabaricus,were examined using reverse transcription polymerase chain reaction (RT-PCR) assay,and NNV was detected in 90% of the rockfishes examined.In infected rockfishes,NNV could be detected in brain and retina most frequently,followed by kidney,less in liver and spleen,but could not be detected in branchia. |
| Key words: Epinephelus spp nervous necrosis virus reverse transcription polymerase chain reaction |
