摘要: |
报道了KCl对方斑东风螺(Babylonia aerolato Link)浮游幼虫变态的诱导作用。结果表明,在水体200 mL,幼虫20只,KCl连续作用12 h的条件下,当KCl浓度不超过8×10-3 mol/L且幼虫日龄小于12 d时,诱导变态率为0~15%,诱导效果不稳定;当KCl浓度达到11×10-3 mol/L且幼虫日龄达到15 d时,浮游幼虫变态率超过95%。在水体850 L,幼虫2.0×105~2.5×105只,KCl连续作用9 h的条件下,KCl浓度为17×10-3 mol/L,日龄15 d的幼虫变态率达90%。浮游幼虫日龄越大,KCl添加浓度越高,作用时间越长,KCl诱导变态作用越明显。KCl对方斑东风螺幼虫有毒性作用,日龄越小,浓度越大,作用时间越长,毒性越大。日龄12 d的浮游幼虫,KCl添加浓度11×10-3 mol/L作用20 h诱导变态的稚螺,未发现其生长和存活受到KCl的不良影响。综合本实验的结果,KCl诱导变态较为安全有效的条件是:浮游幼虫日龄不小于15 d,添加浓度11×10-3~14×10-3 mol/L,作用时间不超过12 h。 |
关键词: 方斑东风螺(Babylonia aerolato) 诱导变态 氯化钾 |
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The effect of KCl on induced metamorphosis of planktonic larvae of Babylonia aerolato |
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Abstract: |
This paper reported the effect of KCl on induced metamorphosis of planktonic larvae of Babylonia aerolato. Under the conditions that the water volume is 200 mL, larva number is 20 and KCl endures 12 hours, the rate of induced metamorphosis is 0~15% and the effect is unstable when the additive concentration of KCl was lower than 8×10-3 mol/L and the age in day of planktonic larvae was younger than 12 days, and the rate of induced metamorphosis was more than 95% when the additive concentration of KCl reached 11×10-3 mol/L and the age in day of planktonic larvae reached 15 day-old. Under the conditions that the water volume is 850 L, larva number is 2.0×105~2.5×105 and KCl endures 9 hours, the rate of induced metamorphosis is 90% when the additive concentration of KCl is 17×10-3 mol/L and the age in day of planktonic larvae is 15 days old. The effect of induced metamorphosis will be more obvious if the age in day of planktonic larvae is older, the additive concentration of KCl is higher and the time that the planktonic larvae last in the additive concentration of KCl is longer. KCl may cause a toxic effect on the larvae of B. aerolato. If the larvae are younger, the concentrations of KCl are higher and the time that the larvae exposed to it is longer, the toxic action will be more serious. The juveniles, which are successfully metamorphosed under the induced conditions, were 12 days old and were exposed to the water with a concentration of 11×10-3 mol/L of KCl for 20 hours, can survive and grow normally in the subsequent cultivations. The summary result of this experiment indicated that the safe and effective conditions for induced metamorphosis are that the age is not younger than 15 days, the additive concentration of KCl was in between 11×10-3~15×10-3 mol/L and the affected time was no longer than 12 hours. |
Key words: Babylonia aerolato induced metamorphosis KCl |