| 摘要: |
| 以大菱鲆(Scophthalmus maximus)出血症的病原菌——鳗弧菌(Vibrio anguillarum)SMP1为抗原,制备兔抗血清,测定了抗血清的工作浓度和对抗原的特异性,建立了在大菱鲆肝肾组织中检测SMP1的间接 ELISA方法。SMP1人工感染大菱鲆,用建立的间接ELISA对大菱鲆肾脏和肝脏进行检测,结果肾脏的阳性检出率为87.7%,肝脏的阳性检出率为 90%。该方法的建立有助于快速准确地鉴定由SMP1引起的大菱鲆疾病。 |
| 关键词: 间接ELISA 鳗弧菌(Vibrio anguillarum) 检测 大菱鲆(Scophthalmus maximus) |
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| 基金项目:国家863计划资助项目(2003AA622070);国家自然科学基金资助项目(30471335);青岛市科技计划项目(04-2-JS-132) |
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| Use of indirect ELISA to detect pathogenic bacterium Vibrio anguillarum SMP1 |
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| Abstract: |
| An indirect Enzyme-Linked Immunosorbent Assay(ELISA) for rapid diagnosis of pathogenic Vibrio anguillarum has been developed. Antiserum specific for the V. anguillarum SMP1 was produced from a New Zealand white rabbit and used in indirect ELISA. The specificity and sensitivity of the antiserum were tested on the liver and kidney tissues of turbot, in which 87.7% out of 30 kidney samples and 90% of liver samples were positive, respectively. |
| Key words: indirect ELISA Vibrio anguillarum detection Scophthalmus maximus |
