| 引用本文: | 赵 宇,李俊卿,张立新,陈 悦,李志萍,陆懋荪,尹佩玉.海蒿子多糖DEI、DEII组分的分离纯化及单糖组成分析[J].海洋科学,2006,30(9):6-8. |
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| 摘要: |
| 海蒿子(Sargassum confusum Agardh)经热水抽提、乙醇沉淀、DEAE-SephadexA-25柱层析分离,得到多糖DEI、DEII,糖质量分数分别为52.40%,38.80%。经酸降解、薄层层析、气相色谱-质谱分析,证明其单糖组成均为岩藻糖、木糖、半乳糖、果糖、甘露糖、葡萄糖。其中岩藻糖质量分数最高(DEI:39.3%; DEII:35.2%)。 |
| 关键词: 海蒿子(Sargassum confusum Agardh) 多糖 分离 纯化 单糖组成 |
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| 基金项目:青岛市科技局资助项目(02-1-kchhh-42-2) |
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| The studies on the isolation, purification, and chemical composition analysis of polysaccharides DEI and DEII from Sargassum confusum |
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| Abstract: |
| The characteristics of the polysaccharide DEI and polysaccharide DEII isolated from Sargassum confusum through water extraction, ethanol precipitation and DEAE-Sephadex A-25 column chromatography sequentially were analyzed by TLC, gel electrophoresis and GC-MS. It was found that these polysaccharides were composed of fucose, xylose, galactose, fructose, mannose and glucose, among them fucose being the primarycomponent (39.3% in DEI and 35.2% in DEII). |
| Key words: Sargassum confusum polysaccharide isolation purification chemical composition |
