| 摘要: |
| 用流式细胞仪分别对日本沼虾 (Macrobrachium nipponense)正常二倍体的体细胞和四倍阶段体胚胎的DNA相对含量和细胞频率进行了测定,以研究日本沼虾染色体倍 性检测技术。以日本沼虾正常二倍体的体细胞液进样所得直方图为标准,用对细胞松弛素B人工诱导的日本沼虾四倍体胚胎进行DNA相对含量和细胞频率的测定, 测试结果以直方图表示,并用染色体涂片镜检结果进行倍性核对。结果表明,日本沼虾二倍体和四倍体的DNA相对含量有显著差异,四倍体日本沼虾的DNA相对 含量是二倍体的2倍。用流式细胞仪法检测日本沼虾倍性,方法简单、测试速度快、结果可靠。 |
| 关键词: 流式细胞仪 日本沼虾(Macrobrachium nipponense) 染色体 倍性检测 |
| DOI： |
| 分类号: |
| 基金项目:浙江省重点攻关项目(2003C22012) |
|
| Detection of cellular DNA content in Macrobrachium nipponense |
|
|
| Abstract: |
| To study the technology of ploidy determination of Macrobrachium nipponense, the flow cytometry was used to measure the DNA relative content and cell frequency of diploid somatic cell and tetraploid embryonic cell from M. nipponense. The results were standardized by histogram. Comparing the DNA relative content and cell frequency between normal diploid somatic cells and tetraploid embryo, which was induced by Cytochalasin B or by cold-heat shocks, from M. nipponense, it was shown that there is a strong correlation between the DNA relative content and ploidy. The DNA relative content in tetraploid embryo was twice as much as that in diploid somatic cells from M. nipponense. It was concluded that using flow cytometry to measure the DNA relative contents is a simple, fast, and reliable method of ploidy determination in M. nipponense. |
| Key words: flow cytometry Macrobrachium nipponense chromosome ploid determination |
