引用本文:	张纹,王军,苏永全.酶联免疫法在贝类麻痹性贝毒检测中的应用[J].海洋科学,2005,29(6):35-37.

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酶联免疫法在贝类麻痹性贝毒检测中的应用
张纹¹, 王军¹, 苏永全¹
厦门大学海洋学系

摘要:

采用酶联免疫法检测菲律宾蛤仔(Ruditapes philippin)肌肉中麻痹性贝毒(Paralytic shellfish poison,PSP)含量,通过优化实验条件,规范操作程序,确立了检测流程。试验表明以标准PSP 为参照,该检测方法平均灵敏度可达2μg/kg,标准溶液测定的变异系数2.00%～7.66%,样品精密度测试的变异系数为2.82%～8.40%,平均添加回收率达85.35%,表现出快速、灵敏、可靠等特点,适于常规工作中麻痹性贝毒的快速筛选检测。

关键词: 麻痹性贝毒酶联免疫法快速检测菲律宾蛤仔(Ruditapes philippin)

DOI：

分类号:

基金项目:厦门市海洋与渔业局资助项目

Enzyme - linked immunosorbent assay(ELISA) quantitative analysis for paralytic shellflsh poison (PSP) in shellflsh

Abstract:

Quantitative analysis of Paralytic Shellfish Poison (PSP) in Ruditapes philippin was carried with Enzyme-Linked Immunosorbent Assay (ELISA). It showed that: the mean detection limit was 2μg/kg, mean recovery rate was 85.35%, and coefficients of variation of 2.82%～8.40% indicated that the screening method is rapid, reliable, and sensitive.

Key words: paralytic shellfish posion ELISA screening method Ruditapes philippin