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| 红鳍东方鲀(Takifugu rubripes)选育家系构建与早期标准化培育 |
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李伟业,马爱军,王新安,孟雪松,刘忠强,刘圣聪,邹 杰,岳 亮,张 涛,熊再峰
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1.中国水产科学研究院黄海水产研究所 农业部海洋渔业可持续发展重点实验室;2.大连天正实业有限公司;3.青岛市海水鱼类种子工程与生物技术重点实验室
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| 摘要: |
| 采用巢式设计方法和人工受精技术, 对红鳍东方鲀(Takifugu rubripes)选育家系的构建与标准化培育技术进行研究。按照1雄配2雌的原则, 选取养殖、野生和日本3个群体的红鳍东方鲀进行定向交配, 并对红鳍东方鲀早期阶段苗种进行了环境标准化和一、二、三、四级数量标准化培育。结果表明, 红鳍东方鲀亲鱼产卵量大, 对构建父系半同胞家系十分有利; 从4次数量标准化看, 每次各家系内鱼苗数量都较为集中, 各阶段各家系的成活率也较高, 但存在部分家系间数量差异显著; 经过4次数量标准化, 成功构建22个父系半同胞家系, 48个母系全同胞家系。对早期阶段家系构建和培育存在的问题, 进行了探讨并提出拟解决方法, 为大规模建立红鳍东方鲀家系提供参照。 |
| 关键词: 红鳍东方鲀 选育 家系构建 标准化培育 |
| DOI：10.11693/hyhz201304017017 |
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| 基金项目:中央级公益性科研院所基本科研业务费专项资金资助, 20603022012005号。 |
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| THE STUDY OF TAKIFUGU RUBRIPES BREEDING FAMILY ESTABLISHMENT AND THE EARLY STANDARDIZED CULTIVATION TECHNIQUE |
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LI Wei-Ye1,2,3, MA Ai-Jun1,4, WANG Xin-An1,4, MENG Xue-Song5, LIU Zhong-Qiang1,4, LIU Sheng-Cong5, ZOU Jie1,4, YUE Liang, ZHANG Tao5, XIONG Zai-Feng5
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1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;2.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture;3.Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology;4.Key Laboratory of Sustainable;5.Dalian Tianzheng Industrial Co. Ltd.
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| Abstract: |
| To carry out a family selective breeding for Takifugu rubripes, a breeding-family program was constructed, and the relevant breeding technology was tested. Three T. rubripes populations, including cultured, wild, and Japanese populations were used in nest mating design at artificial fertilization in 2:1 females : male ratio. At the early breeding stage of T. rubripes, the environment was standardized and the first, second, third and fourth quantity standardization were demanded. The parental fish had large amounts of eggs, which is benefit to the construction of paternal half-sib families. It was found that each family was concentrated in fish number and the survival rate of each family was high. However, a significant difference was found in some families. 22 paternal half-sib families and 48 maternal full-sib families were successfully constructed. Solutions were provided for the problems in family construction and breeding at the early stage, with which a reference was offered to large-scale family breeding program fof T. rubripes. |
| Key words: Takifugu rubripes selective breeding family construction standardized cultivation |