| 摘要: |
| 提取巨蛎属中熊本牡蛎、葡萄牙牡蛎和日本巨牡蛎的基因组 DNA, 应用 PCR 技术扩增了三种牡蛎的海藻糖凝集素结构域(Fucose binding lectin, F-lectin), 结合 GenBank 中长牡蛎海藻糖凝集素结构域的序列利用邻接法对得到的序列进行了系统进化分析, 并利用非同义替换率和同义替换率之间的比值分析了巨蛎属四种牡蛎海藻糖凝集素结构域上可能的受正选择的位点。结果表明, 四种巨蛎属牡蛎的海藻糖凝集素结构域序列中长牡蛎与葡萄牙牡蛎的 F-lectin 比长牡蛎与熊本牡蛎的关系更近, 葡萄牙牡蛎三种单倍型(an1, an2, an3)定位在不同的支上, an1 与长牡蛎海藻糖凝集素结构域的关系更近, an2 与 an3 与熊本牡蛎之间的关系更近。在巨蛎属中, 海藻糖凝集素结构域的 3D 模型中, 发现五个可能的受正向选择的位点(40, 66, 67, 123, 125), 并且这五个位点都围绕在三个重要的识别氨基酸(H37, R64, R70)周围, 推测这五个正向选择位点也许与牡蛎种特异的受精识别有关。 |
| 关键词: 巨蛎属, 结合素蛋白, 海藻糖凝集素结构域, 多样性, 正选择位点 |
| DOI：10.11693/hyhz201102013013 |
| 分类号: |
| 基金项目:国家自然科学基金重点项目, 40730845 号, 国家重点基础研究发展计划(973 项目)资助, 2010CB126401 号; 国家高技术研究发展计划(863 计划)资助, 2010AA1019004 号; 国家公益性行业(农业)科研专项, 3-53 号 |
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| STUDY ON SPERM BINDIN FUCOSE-BINDING LECTIN OF FOUR SPECIES OF CRASSOSTREA |
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WU Qi1,2, LI Li1, ZHANG Guo-Fan1
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1.Institute of Oceanology, Chinese Academy of Sciences;2.Graduate School, Chinese Academy of Sciences
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| Abstract: |
| Gamete recognition proteins (GRPs) play an important role in recognition between sperm and egg. Bindin is a major protein packaged as insoluble granules in sperm acrosomes. Fucose binding lectin (F-lectin) is the main functional domain of bindin. F-lectin repeats of Crassostrea sikamea, C. angulata and C. nippona have been got by polymerase chain reaction (PCR). NJ tree is constructed to analyze the relationship of eleven F-lectin repeats from C. sikamea, C. angulata, C. nippona and C. gigas. Five positive selected positions (40, 66, 67, 123 and 125) are identified among eleven haplotypes of four species. They are all located on the F-lectin binding face around the three recognition motif residues (H37, R64 and R70). We assume that these five clustered amino acids are related with species specific recognition. |
| Key words: Crassostrea, Bindin, Fucose-binding lectin, Polymorphism, Positive selection |
