| 摘要: |
| 以海水硬骨鱼类斜带石斑鱼为研究对象, 通过投喂和腹腔注射半胱胺盐酸盐(CSH), 研究半胱胺对斜带石斑鱼生长激素(GH)及其受体(GHR)mRNA 水平的影响, 以初步揭示半胱胺促进斜带石斑鱼生长的作用机理。结果表明, 长期投喂 CSH 可以显著提高斜带石斑鱼的相对体重增长率。投喂和腹腔注射不同剂量的 CSH 均能明显促进斜带石斑鱼脑垂体 GH mRNA 水平及肝脏 2 种 gGHR mRNA 水平, 且促进效果与 CSH 浓度呈剂量依存关系。本研究还就 CSH 对斜带石斑鱼 2 种不同类型 GHR 作用的剂量效应和时间效应进行了初步探讨。通过注射不同剂量 CSH 发现, 低剂量 CSH(20 μg/g 体重)只可以引起 gGHR2 mRNA 水平的显著提高。中剂量 CSH(50 μg/g 体重)可同时显著提高gGHR1 和 gGHR2 mRNA 水平, 但 gGHR2 水平明显高于 gGHR1 水平; 通过单独注射 50μg/g 体重CSH溶液发现, 注射后2h, 2种gGHR mRNA水平均有显著升高, 其中gGHR2水平明显高于gGHR1。注射后 6h, gGHR2 mRNA 水平率先达到最高值。注射后 12h, gGHR1 mRNA 水平达到最大值。由此可以推测 gGHR2 对于 CSH 所引起刺激的剂量敏感性及时间敏感性均高于 gGHR1。 |
| 关键词: 斜带石斑鱼, 生长, 生长激素, 生长激素受体, 半胱胺 |
| DOI：10.11693/hyhz201102012012 |
| 分类号: |
| 基金项目:上海市教委重点学科项目(海洋生物学), J50701 号; 上海高校选拔培养优秀青年教师科研专项基金, B-8101-08-0012 号 |
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| EFFECTS OF CYSTEAMINE ON mRNA LEVELS OF GROWTH HORMONE AND ITS RECEPTORS IN ORANGE-SPOTTED GROUPER EPINEPHELUS COIOIDES |
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LI Yun, CAI Sheng-Li
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College of Fisheries and Life Sciences, Shanghai Ocean University
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| Abstract: |
| In present research, we found that cysteamine hydrochloride (CSH) administrated in diet could significantly enhance growth of orange-spotted grouper Epinephelus coioides. Both CSH administrated through diet and injection could enhance the levels of growth hormone (GH) mRNA and two types of growth hormone receptors (GHR) mRNA in orange-spotted grouper in a dose-dependent manner. In addition, changes of the levels of two GHRs mRNA induced by the injection of CSH at different doses/hours were investigated. Injection of CSH at 20 μg/g b.w. could only enhance gGHR2 mRNA level significantly; in contrast the medium dose of CSH (50 μg/g b.w.) could significantly enhance two types of GHR mRNA levels. Furthermore, two hours after injection of CSH at 50 μg/g b.w., the levels of two GHR mRNA were increased significantly and gGHR2 mRNA level was much higher than that of gGHR1. gGHR2 mRNA level reached maximum 6 hours after injection, while gGHR1 got to the peak value 12 hours after injection. So, gGHR2 seemed more sensitive to the stimulation induced by the injection of CSH. |
| Key words: Orange-spotted grouper Epinephelus coioides, Growth, Growth hormone, Growth hormone receptor, Cysteamine |
