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斜带髭鲷(Hapalogenys nitens)血细胞发生及外周血细胞化学染色观察
钟爱华, 储张杰, 牟毅, 赵其柱
浙江海洋大学水产学院 舟山 316022
摘要:
通过Wright-Giemsa染色对斜带髭鲷[Hapalogenys nitens(Richardson)]的头肾、体肾、脾脏、肝脏四组织涂片、印片的观察,发现其头肾、体肾、脾脏是其主要的造血器官。头肾能发育生成各类型血细胞,体肾能生成红细胞和粒细胞,脾脏能生成淋巴细胞和红细胞。肝脏中未发现幼稚型血细胞。血细胞发生大致经过原始、幼稚和成熟三个阶段。细胞化学染色显示:外周血细胞中,除淋巴细胞外,均有糖原;嗜中性粒细胞含有ACP、ALP、NACE、αNAE和POX;嗜酸性粒细胞含有ACP、NACE和POX;单核细胞含有ACP、αNAE和POX;小淋巴细胞有ACP。研究发现斜带髭鲷是一种抗病能力较强的鱼类。血细胞组织化学染色可作为斜带髭鲷疾病诊断的辅助手段。
关键词:  斜带髭鲷  血细胞  血细胞发生  细胞化学染色
DOI:10.11693/hyhz20180200044
分类号:S917.4
基金项目:浙江省“水产”一流学科开放课题,20160012号;国家自然科学基金项目,31700322号。
THE HEMOCYTOPOIESIS AND CYTOCHEMICAL STAINING OF PERIPHERAL BLOOD CELL IN HAPALOGENYS NITENS
ZHONG Ai-Hua, CHU Zhang-Jie, MOU Yi, ZHAO Qi-Zhu
Fisheries College of Zhejiang Ocean University, Zhoushan 316022, China
Abstract:
The pronephros, kidney, spleen, and liver of Hapalogenys nitens were observed on stained smears to understand the hemocytopoiesis. The results showed that the three organs were the principal hematopoietic organs. The developments of erythrocyte, granulocyte, lymphocyte, and monocyte were found in mainly the pronephros. The developments of erythrocyte and granulocyte could be found in the kidney and the developments of erythrocyte and lymphocyte could be found in the spleen. There were no immature blood cells in liver smears, indicating no doubt that the liver was not the hematopoietic organ. The development of haemocytes of H. nitens could be divided into three periods, i.e., primitive stage, immature stage, and mature stage. The cytochemical reactions were positive in neutrophils for POX, ACP, ALP, α-NAE, glycogen, and NCAE; in eosnophils for ACP, NACE, POX and glycogen; in monocytes for ACP, POX, glycogen and α-NAE; in lymphocytes for ACP; and in basophils for glycogen. The results showed that H. nitens was a fish with higher disease resistance. The cytochemical approaches utilized here may also have useful applications to studying the diseases affecting this species.
Key words:  Hapalogenys nitens  blood cell  hemocytopoiesis  cytochemical staining
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