| 摘要: |
| 以我国近海重要经济贝类菲律宾蛤仔(Venerupis philippinarum)为对象, 探讨了不同加标浓度Hg2+(2.5、5、7.5、10 μg/L)胁迫下菲律宾蛤仔鳃组织的富集效应, 测定了鳃和血细胞两个组织中过氧化氢酶(CAT)、谷胱甘肽转移酶(GST)、谷胱甘肽过氧化物酶(GPx), 细胞色素P450 (CYP414A1), 硫氧还蛋白(Trx)、金属硫蛋白(MT)、热休克蛋白(HSP70)以及溶菌酶(Lysozyme)8个基因的剂量和时序表达变化, 筛选了指示汞污染的生物标记物。研究结果表明: 蛤仔鳃组织对汞富集随时间延长和Hg2+浓度增加均呈现逐渐上升趋势, 在10 μg/L Hg2+剂量暴露48 h时达到(0.308±0.037) μg/g的最高富集量, 为对照组的21倍(P<0.01)。基因表达分析结果显示, 鳃CYP414A1和GPx基因分别在24 h和48 h的2.5 μg/L暴露组表达显著上调。鳃Lysozyme在24 h的7.5 μg/L处理组中表达显著上调, 鳃HSP70基因在48 h的7.5 μg/L处理组中呈现显著下调趋势(P<0.01)。鳃和血细胞中HSP70基因表达量在10 μg/L处理组中均极显著高于对照组和其它处理组(P<0.01)。鳃CYP414A1和GPx基因、鳃Lysozyme和鳃HSP70、鳃和血细胞HSP70的表达结果可分别作为指示不同浓度汞污染的生物标记物, 其它基因的表达结果不符合生物标记物的筛选原则。研究结果表明组合型生物标记物可较有效地反映海洋环境汞污染状况, 为利用生物标记物进行重金属Hg污染早期监测方法体系的建立提供了基础的研究资料。 |
| 关键词: 菲律宾蛤仔 汞 生物富集 生物标记物 |
| DOI：10.11693/hyhz20150100010 |
| 分类号: |
| 基金项目:浙江省科技厅国际合作项目,2012C24022号;浙江海洋高效健康养殖协同创新中心;浙江省水产种质资源高效利用技术研究重点实验室开放基金,KL2013-4号。 |
附件 |
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| MERCURY BIOACCUMULATION AND BIOMARKERS IDENTIFICATION IN MERCURY EXPOSED VENERUPIS PHILIPPINARUM |
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CHEN Xiao-Cong1, ZHANG Ran1, LI Cheng-Hua1, BAO Yong-Bo2
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1.School of Marine Sciences, Ningbo University, Ningbo 315211, China;2.Zhejiang Wanli University, Zhejiang Key Laboratory of Aquatic Germplasm Resources, Ningbo 315100, China
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| Abstract: |
| We assessed the impact of mercury exposure on Manila clam (Venerupis philippinarum) at four different mock doses (0, 2.5, 5, 7.5 and 10 μg/L) using gill and hemocyte as target.The accumulation of Hg2+in gill was measured by dinatomic fluorescence spectrophotometry.Eight genes expression profiles, including catalase (CAT), glutathione transferase (GST), glutathione peroxidase (GPx), cytochrome P450 (CYP414A1), thioredoxin (Trx), metallothionein (MT), heat shock protein 70 (HSP70) and lysozyme, were further determined by real-time quantitative PCR.Results show that clam gill could accumulate Hg2+in a dose-dependent manner, and the peak value was 21-fold increase over the control group detected at 48 h in 10μg/L exposure dose.CYP414A1 and GPx gene expression significantly increased at 24 h and 48 h in 2.5 μg/L dose of Hg2+, respectively.Lysozyme expression was significantly up-regulated at 24 h in 7.5 μg/L treatment groups in gill, and meanwhile the HSP70 expression trend significantly went downward (P<0.01) at 48 h in 7.5 μg/L exposure.The trend of HSP70 expression in both gill and hemocytes was significantly higher in 10 μg/L group than in other groups.Therefore, CYP414A1 and GPx in gill, lysozyme and HSP70 in gill, HSP70 in gill and hemocytes are potentially good biomarkers to marine mercury monitoring.More importantly, utilization of multiple parameters would greatly improve the accuracy of Hg detection technique.The findings provided basic genomic information to biomonitor mercury pollution by the bivalves in aquatic environment. |
| Key words: Venerupis philippinarum mercury bioaccumulation molecular biomarker |
