| 引用本文: | 李伟萍,胡哲,李玉,王翔,黄文,王宇,肖玲,李水生,张勇,林浩然.斜带石斑鱼(Epinephelus coioides)两种神经肽NPB的发现及表达分析.海洋与湖沼,2015,46(4):879-886. |
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| 斜带石斑鱼(Epinephelus coioides)两种神经肽NPB的发现及表达分析 |
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李伟萍1, 胡哲2, 李玉2, 王翔2, 黄文2, 王宇1, 肖玲2, 李水生2, 张勇2, 林浩然1,2
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1.海南大学海洋学院 海口 570228;2.中山大学水生经济动物研究所暨广东省水生经济动物良种繁育重点实验室 广州 510275
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| 摘要: |
| 神经肽B(Neuropeptide B, NPB)在生命体的许多生理活动中发挥着重要作用, 如调节摄食行为、能量平衡、睡眠、神经内分泌功能和调节炎症性疼痛等。为了研究在石斑鱼中NPB与摄食调控的关系, 本文对斜带石斑鱼(Epinephelus coioides)转录组数据比对分析后, 在斜带石斑鱼中克隆得到了两种NPB(NPB1和NPB2)基因的cDNA序列, 首次发现鱼类存在两种NPB基因。作者对NPB编码蛋白的信号肽及其成熟肽的结构进行了预测, 发现与其它脊椎动物相一致。采用实时荧光定量PCR技术, 研究了斜带石斑鱼不同组织中的mRNA的表达模式, 结果发现NPB1和NPB2在脑组织中广泛表达, 且在下丘脑中表达最为丰富;在其它的外周组织器官中也有相应的表达。饥饿试验的研究结果表明, 在禁食3d和7d后, NPB1和NPB2的mRNA表达量都发生了显著的下调;复投喂后表达量恢复。由此可见NPB1和NPB2参与了斜带石斑鱼摄食行为的调节。 |
| 关键词: 斜带石斑鱼 NPB 基因克隆 组织分布 摄食调控 |
| DOI：10.11693/hyhz20140400098 |
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| 基金项目:国家863计划项目,2012AA10A407号;广东省科技计划项目,2012B090500008号;广州市科技计划珠江科技新星专项,2013J2200093号;广东省海洋渔业科技攻关与研发项目,A201400A01号,A201501A03号,A201501A09号。 |
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| DISCOVERY OF NEUROPEPTIDE B IN GROUPER EPINEPHELUS COIOIDES AND ITS EXPRESSION PATTERN |
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LI Wei-Ping1, HU Zhe2, LI Yu2, WANG Xiang2, HUANG Wen2, WANG Yu1, XIAO Ling2, LI Shui-Sheng2, ZHANG Yong2, LIN Hao-Ran1,2
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1.College of Ocean, Hainan University, Haikou 570228, China;2.Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou 510275, China
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| Abstract: |
| The neuropeptide B (NPB) plays an important role in the regulation of various physiological activities in organisms for regulating feeding behavior, energy balance, sleep, neuroendocrine function, and inflammatory pain.To investigate the function of feeding, we cloned and identified two structures of NPB hypotype genes (NPB1 and NPB2) in the grouper Epinephelus coioides for the first time and compared the transcriptome against the GenBank.NPB1 and NPB2 were found having an open reading frame (ORF) of 393bp and 387bp for encoding a 130 amino-acid (aa) and 128aa-protein sequences, respectively, and having signal peptide and mature peptide structures in the N-terminal regions of the two genes.With real-time quantitative PCR technology, we determined the mRNA expression level of grouper NPB in different tissues.The expression patterns revealed that, NPB1 and NPB2 were widely expressed in brain, especially in hypothalamus, and they were also expressed correspondingly in other peripheral tissues and organs.Our study on food deprivation in the groupers showed that, fasting for 3 and 7 days down-regulated the NPB1 and NPB2 mRNA expression level in the hypothalamus.Therefore, NPB1 and NPB2 are involved in regulating food intake of the groupers. |
| Key words: grouper Epinephelus coioides NPB genetic cloning tissues distribution feeding regulation |
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