引用本文:	贺剑云,何林文,张辛,潘光华,许璞,朱建一,张波,王广策.条斑紫菜(Porphyra yezoensis)遗传多样性的AFLP 分析.海洋与湖沼,2010,41(4):489-494.

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*条斑紫菜(Porphyra yezoensis)遗传多样性的AFLP 分析*
贺剑云¹, 何林文², 张辛³, 潘光华¹, 许璞⁴, 朱建一⁴, 张波¹, 王广策⁵
1.天津科技大学海洋科学与工程学院;2.中国科学院海洋研究所,中国科学院研究生院;3.南京师范大学生命科学学院;4.常熟理工学院生物与食品工程系;5.中国科学院海洋研究所

摘要:

利用扩增片段长度多态性技术(AFLP)分析了 13 个条斑紫菜品系, 在 80 对引物中, 有 11 对能得到重复性好的多态性扩增条带, 共扩增出 619 条谱带, 每对引物扩增带数为 40—77 条, 并且各对引物扩增结果均显示样品间存在差异。共得到 609 条多态性条带, 多态位点的比例高达 98.38%。根据 AFLP 图谱计算了不同样品间的遗传距离(GD)和相似性系数(GS)。聚类结果显示, 品系海安与二室间的遗传相似系数较高而聚合在一起, 不同样品间的相似性系数介于 0.595—0.845 之间。结果表明, 条斑紫菜的遗传多样性极为丰富, 而部分材料间的遗传距离有随地理间距增大而逐渐增大的趋势。聚类分析结果反映了目前条斑紫菜养殖品种间存在着相互混杂。

关键词: AFLP, 条斑紫菜, 遗传多样性, 分子标记

DOI：10.11693/hyhz201004003003

分类号:

基金项目:国家高技术研究发展计划(863计划)资助项目, 2006AA10A413号; 国家科技支撑计划项目, 2006BAD09A04-08号; 国家自然科学基金重点项目资助, 30830015 号。

附件

ANALYSIS OF GENETIC DIVERSITY OF PORPHYRA YEZOENSIS USING AFLP

HE Jian-Yun¹, HE Lin-Wen^2,3, ZHANG Xin⁴, PAN Guang-Hua¹, XU Pu⁵, ZHU Jian-Yi⁵, ZHANG Bo¹, WANG Guang-Ce²

1.College of Marine Science and Engineering, Tianjin University of Science and Technology;2.Institute of Oceanology, Chinese Academy of Sciences;3.Graduate University of Chinese Academy of Sciences;4.College of Life Sciences, Nanjing Normal University;5.College of Biology and Foodengineering, Changshu Institute of Technology

Abstract:

Amplified fragment length polymorphic technique was used to analyze the genomic DNA polymorphism of 13 lines of Porphyra yezoensis. Eleven of the 80 AFLP primer pairs gave reproducible polymorphic DNA amplification patterns, and 619 bands were obtained. The amplified bands ranged from 40 to 77 per primer pair showed the genetic diversity among these samples. Six hundred and nine of AFLP bands were polymorphic loci. The proportion of polymorphic loci was 98.38%. The genetic distance (GD) and similarity coefficients (GS) were calculated based on the polymorphic data. UPGMA cluster indicated that samples from Haian and Ershi were of high similarity. The GS between these samples was 0.595—0.845. The result shows that the genetic diversity level in P. yezoensis is fairly high. Similar increasing trend was found in genetic distance coefficient and geographical distance. UPGMA cluster indicates that P. yezoensis in our country is miscellaneous.

Key words: AFLP, Porphyra yezoensis, Genetic diversity, Molecular marker