| 摘要: |
| 利用扫描和透射电镜技术研究了金乌贼成熟精子的超微结构, 以及超低温冷冻对精子超微结构和形态的影响。结果表明, 金乌贼精子为鞭毛型精子, 外部附有光滑的裙边状被膜, 分为头部、中部和尾部三部分。头部由顶体和细胞核组成, 顶体位于细胞核前端, 呈“囊”状。细胞核为长纺锤状。中部是由线粒体和垂直的中心粒构成, 线粒体大小不一, 多层分布。尾部细长, 轴丝为典型的“9+2”结构。经超低温冷冻的精子被膜上出现很多凹陷; 头部顶体破裂, 大量的内容物流出, 细胞核膜分离; 线粒体解离, 嵴变形; 尾部缠绕在一起, 少数鞭毛出现脱落。
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| 关键词: 金乌贼, 精子, 超微结构 |
| DOI：10.11693/hyhz201004002002 |
| 分类号: |
| 基金项目:国家自然科学基金项目资助, 30600463 号; 中国海洋大学海水养殖教育部重点实验室开放课题资助, 2009008 号; 国家高技术研究发展计划(863 计划)资助项目, 2010AA10A404 号 |
附件 |
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| ULTRASTRUCTURE OF SEPIA ESCULENTA (CEPHALOPODA: SEPIIDA) SPERMATOZOA AND ITS DAMAGE BY LOW TEMPERATURE |
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HAN Song, ZHENG Xiao-Dong, LI Qi, YU Rui-Hai
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The Key Laboratory of Mariculture of Ministry of Education, Ocean University of China
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| Abstract: |
| Electron microscopy was used to study the morphologies and ultrastructures of the spermatozoa of golden cuttlefish Sepia esculenta before and after low temperature treatment. The flagellum-like sperm of S. esculenta, covered by a smooth membrane, consisted of a head, a middle piece and a tail. The head comprised a bubble-shaped acrosome and an ellipse nucleus, with the acrosome located in the front of the nucleus. The middle piece contained many mitochondria and vertical central granules. The mitochondria were uneven in size and overlappingly arrayed. The tail was long and thin, with the typical “9+2” structure. After the storage at the low temperature of ?80℃, there were many holes on the membrane of the head of spermatozoa. The acrosome was fractured, losing its inclusion. Karyotheca was separated from the nucleolus. Mitochondria dropped off, and the inside membrane became deformed. Sperm tails were intertwined together; and some flagella were broken and cast off.
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| Key words: Sepia esculenta, Spermatozoon, Ultrastructure |
