| 摘要: |
| 1990年以来，采用多因子正交试验方法，对悬浮生长的条斑紫菜膨大藻丝即孢了囊枝生长发育规律进行研究。通过细胞工程方法，建立悬浮培养的膨大藻丝无性系，并使该无性系长年大量繁殖。经过对膨大藻丝的生长发育调控，可使膨大藻丝适时、大量放散壳孢子。 |
| 关键词: 条斑紫菜 膨大藻丝无性系 悬浮培养 |
| DOI： |
| 分类号: |
| 基金项目:“九五”攻关预研究项目，953076号 |
附件 |
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| PRELIMINARY REPORT ON SUSPENSION CULTURE OF CLON OF PORPHYRA YEZOENSIS CONCHOSPORANGIAL FILAMENTS IN THE PRODUCTION OF CONCHOSPORES FOR PURPLE LAVER AQUACULTURE |
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Sun Aishu, Zeng Chengkui (C. K. Tseng)
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Institute of Oceanology, Chinese Academy of sciences, Qingdao 266071
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| Abstract: |
| For the aquaculture of Porphyra, seedling is a key link. At present, carpospores of the Porphyra are sown on molluscan shells and the Conchocelis stage is produced. The latter gives rise to the concho sporangial filaments, finally produces conchospores. Production nets are provided on which the liberated conchospores attach and grow. The whole process takes 5 - 6 months, and a large area of ponds have to be devoted to culture of the Conchocelis in shells. Iwasaki et al. (1963) reported on cultivation of the free—living Conchocelis. But it still had to be sown on molluscan shells and cultivated in ponds. It seems that only the Conchocelis growing in shells are capable of producing conchospores. At present similar method is employed in China. Therefore it is of great importance whether suspension cultivation of concho—sporangial filaments without the shells are able to produce conchospores.
In Spring of 1990, carpospores of Porphyra yezoensis were collected, germinated and grew up to the free—living Conchocelis, which produced conchosporangial filaments. All experiments were conducted in culture boxes with light and temperature control. The results of a number of experiments are as follows:
1. After the development of concho—sporangial filaments, they can be cultivated alone, and normally develop and discharge a large number of conchospores.
.2. The suspension conchosporangial ?lament used in present work have been vegetatively propagated many times in several years. They are able to liberate a great quantity of conchospores. This means the shells are not indispensable for the development of conchosporangial filament and discharge of conchospores. Similar to the Conchocelis filements in the shells, the suspension concho—sporangial filaments can form and discharge conchospores.
3. The clon used in the present work have been asexually propagated at least 4 to 5 times a year, for more than 6 years. It shows that the concho—sporangial filaments can be propagated indefinitely and that a good clon can be preserved for a very long time.
4. The clon selected in 1990 produced numerous conchospores (P1. I: 1, 2). Some of these were collected by adhering to nylon nets, and sporeling thread and normally grew to sporelings (P1. I: 3, 4). Each gram (fresh weight) of the cultured concho—sporangial filaments discharged as much as 1000 million conchospores per day.
The breeding technique by cell engineering presented above involves culture of suspension Conchocelis phase and through adjusting necessary facts for their development producing conchosporangial filaments. A clon of the concho—sporangial filaments was set up and their mass vegetative propagation and artificial control for forming and discharging conchospores . The conchospores are then collected and cultivated to sporeling. Since no shells are used as the culture substrates and mass propagation of the concho—sporangial filaments in bioreactors will be possible. This will thoroughly change traditional breeding way. |
| Key words: Porphyra yezoensis, Clon of concho-sporangial filaments, Suspension culture |
