引用本文:	秦松,童顺,崔武,李亚菲,王希华,武建秋,吴光耀,张培军,曾呈奎.钝顶螺旋藻质粒的电镜观察及杂交研究.海洋与湖沼,1994,25(5):560-563.

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钝顶螺旋藻质粒的电镜观察及杂交研究
秦松¹, 童顺¹, 崔武², 李亚菲³, 王希华¹, 武建秋¹, 吴光耀², 张培军¹, 曾呈奎¹
1.中国科学院海洋研究所青岛 266071;2.北京大学生物系北京 100871;3.中国人民解放军肝病研究所北京 100700

摘要:

于1992年6月－1993年4月，对钝顶螺旋藻质粒进行透射电镜观察及分子杂交研究。结果表明，利用单层分子膜展开技术，能够观察到超螺旋以及闭合环形的质粒图象；S₆质粒大小的平均值约为2.0kb，与电泳检测的结果稍有出入。斑点杂交结果表明，不同形态藻株S₆、F₃、F₃弯与F₃直质位之间具有一定同源性，与染色体DNA之间均显示同源性。对钝顶螺旋藻质位的进一步研究将推动其基因工程育种的开展。

关键词: 蓝藻钝顶螺旋藻质粒

DOI：

分类号:

基金项目:中国科学院海洋研究所调查研究报告第2338号。中国科学院院长基金部分资助，中国科学院海洋研究所所长基金资助

附件

TEM OBSERVATION AND HYBRIDIZATION STUDY OF PLASMID FROM SPIRULINA PLATENSIS

Qin Song¹, Tong Shun¹, Cui Wu², Li Yafei³, Wang Xihua¹, Wu Jianqiu¹, Wu Guangyao², Zhang Peijun¹, Zeng Chengkui (C. K. Tseng)¹

1.Institute of Oceanology Academia Sinica, Qingdao 266071;2.Department of Biology, Beijing University, Beijing 100871;3.Institute for Liver Diseases, PLA, Beijing 100700

Abstract:

Transmission electron microscopic observation and hybridization study of the plasmid from Spirulina platensis were carried out from June, 1992 to April, 1993. Supercoiled and closed circular patterns were observed under a transmission electron microscope. The size of the plasmid from S₆ strain was about 2.0kb, which was slightly different from the result of electrophoresis. Results of dot blotting show that plasmids from strains of S₆, F₃, F₃ spiral and F₃ straight differing in form are homologous to each other and are also homologous to chromosomal DNAs.

Key words: Cyanophyte, Spirulina platensis, Plasmid