| 摘要: |
| 毛细管电泳技术是近年来逐步发展、完善的一项新的分析检测手段。其中区带毛细管电泳技术应用得较为普遍。由于所需样品量少，操作简便、快速，检测灵敏，因此毛细管电泳技术被广泛用来分析和分离多肽，蛋白质，糖蛋白，寡聚核酸等物质(Siles et al.，1997; Leeds et al.，1996)。在国外已开始用区带毛细管电泳技术来研究免疫球蛋白的稳定性(Dai et al.，1998)，分析人血清蛋白的组成，并尝试作为临床诊断手段(Voelter et al.，1998)，但这一技术在海洋科学研究领域还未获得应用。通常鱼类血清中蛋白质的含量以及蛋白组分的变化，可以用来判断鱼体免疫功能的水平。用区带毛细管电泳方法来检测蛋白质样品，不但能够检测出样品的种类，同时能够较准确地检测出各种蛋白质的含量，是一种理想的方法。本文作者第一次试用毛细管电泳技术分析牙鲆血清蛋白的组成，并比较了在一定条件下血清蛋白组成的变化。 |
| 关键词: 毛细管电泳 血清蛋白 牙鲆 |
| DOI： |
| 分类号: |
| 基金项目:中国科学院海洋研究所调查研究报告第3847号。国家973课题“鱼类的特异性免疫系统和疫苗免疫”资助项目，G19999012006号。 |
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| ANALYSIS OF THE SERUM PROTEIN COMPONENTS OF FLOUNDER, PARALICHTHYS OLIVACEUS WITH CAPILLARY ZONE ELECTROPHORESIS (CZE) |
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Wang Hongtian, Zhang Peijun
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Institute of Oceanology, Chinese Academy of Sciences
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| Abstract: |
| The serum protein components of flounder, Paralichthys olivaceu were analyzed by capillary zone electrophoresis (CZE). Three kinds of proteins can be analyzed with current experimental conditions. Based on the time that these proteins appeared, they were named as Peak 2.59, Peak 1.60 and Peak 0.77. After storage at room temperature (25℃) for 24 hours, the protein Peak 2.59 remained stable, while the other two proteins were destroyed. Comparison of the results with those of the analysis of human beings serum, the protein Peak 2.59 is probably attributable to Albumin, the protein Peak 1.60 is probably attributable to Globulin-α, the protein Peak 0.77 is probably attributable to Globulin-β. |
| Key words: capillary zone electrophoresis serum protein |
