| 摘要: |
| 碱性磷酸酶(alkaline phosphatases)是一种内源性酶，几乎存在于所有动物的细胞膜(plasma membrane)中，它在许多代谢活动中，如动物的生长、蛋自质的合成、营养成分的转运、类固醇的生成等方面都发挥着重要的作用(Ram et al.，1985)。因此，对于碱性磷酸酶的研究，有利于了解鱼类的消化生理，并有助于更深刻地理解鱼类体内营养成分的吸收和转运机制。假雄牙鲆是在雌性牙鲆的一定生长发育阶段，用人工诱导的方法使之性转化所获得的。对于假雄牙鲆的生理、生化特性的研究，至今还未见正式报道。本实验以一年龄假雄牙鲆为研究对象，研究了不同消化器官中碱性磷酸酶的比活性，并初步探讨了在不同发育时期碱性磷酸酶比活性的变化，从而能够更深刻地理解假雄牙鲆体内营养成分消化与吸收的过程和机制，并有助于牙鮃的人工培育。 |
| 关键词: 碱性磷酸酶 假雄牙鲆 比活性 |
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| 基金项目:中国科学院海洋研究所调查研究报告第3846号。国家863课题“全雌牙鲆遗传育种研究”资助项目，863-819-01-03号。 |
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| THE SPECIFIC ACTIVITIES OF ALKALINE PHOSPHATASE IN DIFFERENT DIGESTIVE TISSUES OF PSEUDOMALE BASTARD HALIBUT PARALICHTHYS OLIVACEUS |
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Wang Hongtian, Xu Yongli, Zhang Peijun
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Institute of Oceanology, Chinese Academy of Sciences
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| Abstract: |
| The specific activities of alkaline phosphatase in different digestive tissues in one-year-old pseudomale Paralichthys olivaceus were studied, and the enzyme activities in different stages were compared. The mean weights of the fish in Group I was 350±20g. The specific activities of alkaline phosphatase in foregut, midgut, hindgut, liver, and stomach were 0.097±0.015, 0.089±0.017, 0.068±0.011, 0.072±0.015, 0.037±0.0l6 (unit/(min?mg)).The mean weights of the fish in group was 460±30g. The specific activities of alkaline phosphatase in the foregut, midgut, hindgut, liver, stomach were 0.135±0.026, 0.128±0.019, 0.095±0.012, 0.101±0.017, 0.064±0.011 (unit/(min?mg)). From the results it can be concluded that the specific activities of alkaline phosphatase in different digestive tissues of pseudomale Paralichthys olivaceus are different. The specific activities of the alkaline phosphatase in the foregut and midgut are higher than those in other tissues, while the enzyme specific activity in the stomach is the lowest. Additionally, the enzyme activities in the liver and different parts of the gut of the fish in Group Ⅱ are higher than those in Group Ⅰ, while no significant difference was observed in the stomach. |
| Key words: alkaline phosphatase specific activities pseudomale Paralichthys olivaceus |
