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甲壳素提取方法的研究进展
周忆1,2, 邢荣娥1,3,4, 杨皓月1,4, 刘松1,3,4, 李鹏程1,3,4
1.中国科学院海洋研究所 实验海洋生物学重点实验室, 山东 青岛 266071;2.中国科学院大学, 北京 100049;3.青岛海洋科学与技术国家实验室 海洋药物与生物制品功能实验室, 山东 青岛 266237;4.中国科学院海洋大科学研究中心, 山东 青岛 266071
摘要:
甲壳素是一类重要且应用广泛的天然高分子。海洋甲壳类加工副产物, 如虾壳、蟹壳等, 是甲壳素的重要来源之一, 常见的提取方法主要有化学法、酶法、微生物发酵法等, 其中微生物发酵法是一种依赖微生物发酵完成脱矿(DM)、脱蛋白(DP)过程的方法, 整个过程不引入任何化学试剂, 绿色无污染, 是解决目前甲壳素生产过程中酸、碱用量大导致污染严重的关键技术。本文综述了各种甲壳素提取方法及各种影响因素在提取过程对所得甲壳素性质的影响, 讨论了微生物发酵法的相关内容, 以期为相关研究提供理论支撑。
关键词:  甲壳素  提取方法  微生物发酵
DOI:10.11759/hykx20220905001
分类号:
基金项目:中国科学院海洋大科学研究中心重点部署项目(技术研发类)(COMS2020J04);山东省重点研发计划(重大科技创新工程)项目(2022CXGC020413);国家重点研发计划(2018YFC0311305)
Research progress on chitin extraction methods
ZHOU Yi1,2, XING Rong-e1,3,4, YANG Hao-yue1,4, LIU Song1,3,4, LI Peng-cheng1,3,4
1.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.National Laboratory of Marine Science and Technology, Qingdao 266237, China;4.Marine Research Center, Chinese Academy of Sciences, Qingdao 266071, China
Abstract:
Chitin is an important and widely used natural polymer. Byproducts obtained during the processing of marine crustaceans, such as shrimp and crab shells, are an important source of chitin. Commonly used chitin extraction methods include chemical, enzymatic, and microbial fermentation methods. Among them, the microbial fermentation method relies on microbial fermentation for the completion of demineralization and deproteinization processes. The entire process does not involve the use of chemical reagents, making it a green and pollution-free method. This article reviews various chitin extraction methods, factors affecting its extraction, the effect of the extraction process on the properties of chitin, and discusses some relevant contents of microbial fermentation hoping to provide a theoretical foundation for related research.
Key words:  chitin  extraction methods  microbial fermentation
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