| 摘要: |
| 建立RP-HPLC （反相高效液相色谱法）同时测定翅碱蓬中四种黄酮化合物-芸香苷、槲皮素3-β-D-葡萄糖苷、水仙苷和槲皮素的含量。采用Kromasil C18色谱柱（4.6 mm×250 mm，5 μm），检测波长为361 nm，柱温为30℃，流动相为甲醇-0.4%冰乙酸（55：45），流速1.0 mL/min，四种黄酮类化合物得到很好的分离。芸香苷、槲皮素3-β-D-葡萄糖苷、水仙苷和槲皮素浓度均为0.001~0.01 mg/mL范围内呈现良好线性关系，相关系数R2在0.999 0~0.999 6，加标回收率（n=3）范围为82.39%~99.03%。该方法简单、快速、高效，可以用于翅碱蓬中四种黄酮化合物的含量分析。 |
| 关键词: 翅碱蓬 反相高效液相色谱 芸香苷 槲皮素3-β-D-葡萄糖苷 水仙苷 槲皮素 |
| DOI：10.11759/hykx20190716003 |
| 分类号:O629.9 |
| 基金项目:辽宁省自然科学基金重点项目（20170540102），盘锦红海滩湿地退化与生态修复项目（PHL-XZ-2017013002） |
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| Simultaneous determination of four flavonoids in Suaeda Heteroptera by RP-HPLC |
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GUO Li-xia, NING Huan-yu, CAO Wei, LI Hui, KONG Liang, TAN Cheng-yu
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College of Marine Science and Environment, Dalian Ocean University, Dalian 116023, China
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| Abstract: |
| In this study, we established a reversed-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous determination of four flavonoids—rutin, quercetin 3-β-D-glucoside, narcissoside, and quercetin—in Suaeda Heteroptera. The separation was achieved using a Kromasil C18 column (4.6 mm×250 mm, 5 μm) at 30℃, with methanol-0.4% glacial acetic acid (55︰45) as the mobile phase at a flow rate of 1.0 mL.min-1 and a detection wavelength of 361 nm. The correlation coefficients ranged between 0.999 0 and 0.999 6 with calibration curves in the range of 0.001-0.01 mg·mL-1 for the four flavones. The average recoveries (n=9) obtained were 82.39%-99.03%. This method is simple, rapid, and efficient, and can be used for the simultaneous determination of rutin, quercetin 3-β-D glucoside, narcissoside, and quercetin in Suaeda Heteroptera samples. |
| Key words: Suaeda Heteroptera RP-HPLC flavonoids rutin quercetin 3-β-D-glucoside narcissoside quercetin |
