| 摘要: |
| 通过研究硝基苯和间苯二酚对海洋微藻的毒理效应, 旨在提示化工废水污染对水环境破坏的严重性, 揭示海洋微藻类净化废水的潜在应用。本试验用塔玛亚历山大藻(Alexandrium tamarense)、海链藻(Thalassiosira weissflogii)和绿色巴夫藻(Pavlova viridis)为供试藻种, 以微藻生长量及叶绿素a 的变化为参数, 研究不同质量浓度的硝基苯和间苯二酚对3 种海洋微藻的毒理效应。结果表明: 硝基苯对塔玛藻和巴夫藻的生长具有一定的抑制作用, 质量浓度为12mg/L 时抑制率分别为22.9%和20.04%, 但对海链藻的生长在一定程度上有促进作用。间苯二酚对海链藻和巴夫藻的生长具有抑制作用, 质量浓度为12mg/L 时其抑制率分别为26.11%和23.6%, 对塔玛亚历山大藻的生长有一定的促进作用。对塔玛亚历山大藻和巴夫藻而言, 在硝基苯质量浓度为3mg/L 时, 叶绿素a 含量均达到最低, 其叶绿素a含量分别为对照组的68.6%和50.9%, 对于海链藻, 硝基苯对其叶绿素a 的影响波动不大。间苯二酚对3 种海洋微藻叶绿素a 含量的影响为绿色巴夫藻>海链藻>塔玛亚历山大藻, 其叶绿素a 含量分别是对照组的1.1%, 24.9%, 88%。 |
| 关键词: 硝基苯 间苯二酚 海洋微藻 叶绿素a 毒理效应 |
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| 基金项目:国家科技部“十二五”科技支撑项目(2011BAD13B03); 江苏省科技厅苏北科技发展计划项目(BN2011035) |
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| The toxicological effects of nitrobenzene and dihydroxybenzene on three kinds of marine microalgae |
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| Abstract: |
| The investigation of toxicological effects of nitrobenzene and dihydroxybenzene on marine microalgae was designed to tip the severity of damage by chemical wastewater pollution in the aquatic environment and revealed potential application of marine microalgae in the purification of wastewater. Using the Alexandrium tamarense, Thalassiosira weissflogii and Pavlova viridis as tested species, microalgae growth and chlorophyll a changes as parameters, the toxicological effects of different concentrations of nitrobenzene and dihydroxybenzene on these three kinds of marine microalgae were studied. The results showed that nitrobenzene had inhibitory effect on the growth of A.tamarense and P. viridis and the inhibition rates were 22.9% and 20.04%, respectively at the concentration of 12 mg/L, but had stimulative effect on the growth of T. weissflogii in some extent. Dihydroxybenzene had inhibitory effect on the growth of T. weissflogii and P. viridis and the inhibition rates were 26.11% and 23.6% at the concentration of 12 mg/L, but had a stimulative effect on the growth of A. tamarense. The content of chlorophyll a in the A. tamarense and P. viridis reached the minimum at the nitrobenzene concentrations of 3 mg/L, and they were 68.6% and 50.9% of the control group respectively, but less effect on that of T. weissflogii. Dihydroxybenzene had some effect on the content of chlorophyll a in the three kinds of marine microalgae: P. viridis>T. weissflogii>A. tamarense, with chlorophyll a concentration of 1.1%, 24.9%, 88% respectively of the control group. |
| Key words: nitrobenzene dihydroxybenzene marine microalgae Chlorophyll a Toxicological effect |
