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泥蚶初级卵母细胞发育及卵黄发生的超微结构研究
顾海龙1,2, 林志华2, 沈伟良3, 陈彩芳2
1.上海海洋大学 水产与生命学院;2.浙江万里学院 生物与环境学院;3.宁波市海洋与渔业研究院
摘要:
利用透射电镜技术观察了泥蚶(Tegillarca granosa)初级卵母细胞发育期间各细胞器的特征及卵黄粒的形成过程。结果显示: 泥蚶初级卵母细胞的发育过程可以分为卵黄合成前期、卵黄合成期及卵黄合成后期三个时期。在卵黄发生过程中, 线粒体、高尔基体、粗面内质网、微吞饮泡等都参与了卵黄粒的形成, 且线粒体和粗面内质网是形成卵黄粒的主要胞器。卵黄发生的原料以外源性合成为主,外源性营养物质主要来自消化腺。
关键词:  泥蚶(Tegillarca granosa)  卵黄发生  超微结构  初级卵母细胞
DOI:
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基金项目:国家贝类产业技术体系项目(CARS-48); 宁波市科技创新团队项目(2011B82017); 宁波市自然科学基金项目(2011A610016)
Ultrastructural studies on the development of primary oocytes and vitellogenesis of Tegillarca granosa
Abstract:
The ultrastructural features of the oogenesis and the formation of yolk granules of Tegillarca granosa are using transmission electron microscope. The primary oocyte developmental process is divided into three stages: previtellogenic stage, vitellogenic stage and postvitellogenic stage. Mitochondria, golgi complex, rough endoplasmic reticulum and micro-pinocytosis bubble all participate in the formation of yolk granules and the rough endoplasmic reticulum and mitochondria are key organelles in this process. The heterosynthetic process is the main form of oogenesis, and the exogenous nutrients are mainly from the digestive gland.
Key words:  Tegillarca granosa  vitellogenesis  ultrastructure  primary oocyte
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