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| 海洋硫酸多糖几种脱硫方法的比较研究 |
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刘 鑫1,2, 王玉峰1,2, 曾洋洋1,2, 王培培1,2, 韩章润1,2, 吴建东1,2, 于广利1,2
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1.海洋药物教育部重点实验室;2.山东省糖科学与糖工程重点实验室, 中国海洋大学医药学院
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| 摘要: |
| 以κ-卡拉胶(硫酸基含量25.1 %, 分子质量83.1 kD)为原料, 研究了二甲基亚砜-甲醇法(DMSO法)、三甲基氯硅烷法(CTMS 法)以及苯并四甲酸-三氧化二锑法(PMA 法)对其脱硫后多糖样品的得率、硫酸基含量及分子质量的影响。结果表明, κ-卡拉胶经三种方法脱硫后, 样品得率分别为45.3 %、50.6 %和62.0%, 脱硫率分别为42.2 %、48.6 %和 82.8 %, 分子质量分别为15.7、22.3 和4.75 kD。通过红外光谱和硫酸基含量分析表明, PMA 法脱硫效果优于DMSO 和CTMS 法。PMA 法不仅对κ-卡拉胶有效,而且适合于ι-卡拉胶、λ-卡拉胶和岩藻聚糖硫酸酯的脱硫。 |
| 关键词: 海洋硫酸多糖 卡拉胶 脱硫 苯并四甲酸 红外光谱 |
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| 基金项目:国家自然科学基金项目(31070724); 国家海洋局公益性行业科研专项项目(201005024); 长江学者和创新团队发展计划项目(IRT0944) |
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| Comparison of several desulfation methods of marine sulfated polysaccharide |
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| Abstract: |
| κ-carrageenan (sulfate content 25.1 %, molecular mass 83.1kD) was selected as studying material. The yields, sulfate contents and molecular masses of desulfated products of the methods of DMSO-MeOH (DMSO), trimethylchlorosilane (CTMS) and pyromellitic acid-Sb2O3 (PMA) were compared. The results showed that the yields of the desulfated κ-carrageenan were 45.3 %, 50.6 % and 62.0 %, the desulfation rates were 42.2 %, 48.6 % and 82.8 %, the molecular masses were 15.7 kD, 22.3 kD and 4.75 kD, respectively. According to the IR spectrum and sulfate content analysis, PMA method was better than the DMSO and CTMS method. PMA method was not only effective for κ-carrageenan, but also suitable for the desulfation of ι-carrageenan, λ-carrageenan and fucoidan polysaccharide. |
| Key words: Marine polysaccharide carrageenan desulfation pyromellitic acid IR |