| 摘要: |
| 采用平板涂布法, 以青岛海区潮间带沉积物为对象进行海洋放线菌的分离方法研究。具体分析了不同样品预处理方式、稀释液、海水浓度和培养基种类等对分离效果的影响。结果表明, 55℃预处理样品6 min 能有效减少细菌数量, 利于潮间带沉积物中放线菌的分离; 以1/4 林格氏溶液稀释样品、纯海水配置培养基, 可以分离得到较多的放线菌; 不同培养基对沉积物中放线菌的分离效果差别很大,本实验设置的M1～M9 培养基中, M1、M6、M7 和M8 培养基的分离效果优于其他5 种。 |
| 关键词: 海洋沉积物 放线菌 分离方法 |
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| 基金项目:国家高技术研究发展计划资助项目(2007AA09Z434); 国家海洋局海洋生物活性物质与现代分析技术重点实验室开放基金资助项目(MBSMAT-2010-02); 烟台大学博士启动基金资助项目(HJ11B26) |
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| Comparative study of different methods for isolation of marine actinomycetes |
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| Abstract: |
| The isolation techniques for marine actinomycetes from the inter-tidal sediment at Qingdao were studied by using the series dilution and plate spreading methods. The impacts of different pretreatments, diluents, seawater concentrations and media on the isolation of actinomycetes were investigated. The results showed that the growth of bacteria were obviously restrained in the samples when pretreated with 55°C for 6 minutes, which enhanced the isolation of actinomycetes from the inter-tidal sediment; dilution of the samples with 1/4 Ringer's solution and spreading them on the media prepared with pure seawater could improve the isolation of actinomycetes. The 9 media exhibited significant differences on the number of actinomycetes recovered, with media M1, M6, M7 and M8 being more effective than others. |
| Key words: marine sediment actinomycete isolation method |
