| 摘要: |
| 利用AFLP 技术对舟山近海条石鲷(Oplegnathus fasciatus)野生和放流群体的遗传多样性进行比较分析, 旨在为条石鲷的人工增殖及其种质资源的保护和利用提供遗传学的基础资料。采用8 对引物组合在2 个群体中共扩增得到位点316 个, 多态性位点为162 个, 多态性比例为51.67%。野生群体和放流群体的多态性位点比例(P), Nei’s 基因多样性指数(H)和Shannon’s 多样性指数(I)分别为46.75%和44.67%, 0.097 和0.089, 0.16 和0.15。野生群体的遗传多样性水平略高于放流群体, 但差异并不显著(P>0.05)。两群体间的遗传相似系数(S)和遗传距离(D)分别为0.99 和0.0073; 基因分化系数(Gst)为0.036,群体间无显著遗传分化(P>0.05)。分子方差(AMOVA)分析表明96.82%的遗传变异来源于群体内的个体间, 群体间无显著的遗传差异(P>0.05)。以上研究结果表明条石鲷的放流群体与野生群体间无明显的遗传分化, 放流群体的遗传多样性水平尚处于一个合理状态。但为了避免放流群体对野生群体产生负面的遗传效应, 应当增加放流群体繁育亲本的数量, 并对放流群体的遗传变异水平进行持续监测。 |
| 关键词: 条石鲷(Oplegnathus fasciatus) 野生群体 人工放流群体 遗传多样性 AFLP |
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| 基金项目:国家科技支撑计划资助项目(2011BAD13B08); 国家海洋公益性行业科研专项(201005013); 国家自然科学基金资助项目(41106114); 浙江省科技计划资助项目(2010F20006); 浙江省海水养殖重点科技创新团队项目(2010R50025) |
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| The genetic diversity of wild and hatchery-released Oplegnathus fasciatus from inshore water of Zhoushan revealed by AFLP |
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| Abstract: |
| The genetic variations of wild and hatchery-released Oplegnathus fasciatus from inshore water of Zhoushan were analyzed using amplified fragment length polymorphism (AFLP) to provide basic genetic information for the population enhancement and genetic resource protection and utilization in this fish species. A total of 316 loci were generated in the two populations using 8 primer combinations, among which 162 (51.67％) were polymorphic. The percentages of polymorphic loci in the wild or hatchery-released populations were 46.75% or 44.67%, respectively. The genetic diversities in terms of Nei’s gene diversity and Shannon’s diversity index in the wild population were 0.097 and 0.16, respectively. The corresponding values in hatchery-released population were 0.089 and 0.15, respectively. These results showed that the genetic diversity in the wild population was slightly higher than that in the hatchery-released population, but the difference in genetic diversity of the two populations was not significant (P>0.05). The genetic similarity coefficient and genetic distance between the two populations were 0.99 and 0.0073, respectively. The value of Gst coefficient was 0.036, which indicated no significant genetic differentiation between the two populations (P>0.05). The analysis of molecular variance (AMOVA) showed that 96.82% of the genetic variation occurred among individuals within populations, which also revealed no significant genetic variation between the two populations (P>0.05). The genetic diversity of hatchery-released population was at a reasonable level of state and genetic differentiation was not significant between the wild and hatchery-released populations. It was noticeable to take approaches to avoid the potential negative genetic effects of hatchery-released on wild populations. The number of parental fish should increase and the genetic variation level of hatchery-released population ought to be continuously monitored in the future. |
| Key words: Oplegnathus fasciatus wild population hatchery-released population genetic diversity AFLP |
