| 摘要: |
| 分别研究了徐闻3 种滨珊瑚的ITS1 和ITS2 基因的碱基组成和G+C 含量, 并和已上传至Genbank上的其他9 种滨珊瑚的ITS 序列进行比较, 研究了徐闻3 种滨珊瑚的系统发生关系。序列分析结果显示, 3 种滨珊瑚的ITS1 的长度为205 bp～209 bp, G+C含量为37.6%～45.5%, ITS2 的长度为192 bp～226 bp,G+C 含量为45.1%～50.7%, 利用MEGA4.1 软件计算滨珊瑚属基于ITS1 和ITS2 基因的平均遗传距离分别为0.097 和0.200。基于ITS1 和ITS2 的NJ 系统进化树都显示出, 灰黑滨珊瑚位于进化树的基部,是原始的类群, 普格滨珊瑚是进化类群, 澄黄滨珊瑚是过渡类群。 |
| 关键词: 滨珊瑚 ITS 基因 系统发生关系 |
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| 基金项目:广东省海洋渔业科技推广专项(A200908F01); 国家海洋公益性行业科研专项(201105012) |
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| Phylogeny analysis of Porites link in Xuwen using its gene |
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| Abstract: |
| The obtained ITS (internal transcribed spacer) sequences of 3 species in Porites by PCR technique were analyzed by MEGA4.1 software to get base composition, G+C content and phylogenetic relationship with ITS gene of other 9 species uploaded to Genbank. The results showed that the ranges of ITS1 and ITS2 of the tested 3 species were 205～209 bp and 197～226 bp, and the ranges of G+C content of ITS1 and ITS2 were 37.6%～45.5% and 45.1%～50.7%. The average genetic distance based on ITS1 and ITS2 were 0.097 and 0.200 respectively. The NJ-based phylogenetic tree based both ITS1 and ITS2 shows that Porites nigrescens at the base of evolutionary tree, is a primitive group, Porites pukoensis is an evolutionary group and Porites lutea is a transitional group. |
| Key words: porites ITS genes phylogeny |
