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Te(IV)对螺旋藻胁迫作用的光谱学研究
杨 芳1,2, 涂 芳1, 刘 杰1, 郑文杰1,2
1.暨南大学 化学系;2.水体富营养化与赤潮防治广东省教育厅重点实验室
摘要:
用光谱法研究了Te(IV)对螺旋藻(Spirulina maxima)的胁迫作用。各实验组添加Te(IV)的时间均在第5~7 天, 累积添加质量浓度为从450~650 mg/L。结果表明, 各Te(IV)胁迫实验组中活藻体悬浮液的吸收光谱特征峰强度均不同程度地下降。而在440、580 nm 激发所得的荧光发射光谱峰位置不变, 峰强度下降。但干粉的荧光光谱与活藻体悬浮液有明显不同, 干粉中的藻胆体的能量传递过程受阻。各试验组干藻粉的红外光谱无明显差异。
关键词:  碲胁迫  螺旋藻(Spirulina maxima)  光谱特性
DOI:
分类号:
基金项目:国家自然科学基金项目(20771044); 广东省科技计划项目(2007B030703007, 2008A030201020); 中国博士后基金项目(20090460787); 中央高校基本科研业务费专项资金(21610303)
Spectrum Study of Te(IV) stress on the growth of Spirulina maxima
Abstract:
The effect of Te(IV) stress on the growth of Spirulina maxima has been studied via spectrum analysis. The Te(IV) adding time ranged from the 5th to 7th day and the accumulating Te(IV) concentration was from 450 to 650 mg/L. The absorption spectra showed that the intensity of typical absorption peak of S. maxima aqueous suspension decreased in the presence of Te(IV) stress. The fluorescence emission peaks which were excited at 440 and 580 nm showed that the intensity of peaks decreased in vivo. In contrast, the IR spectra of different groups have no significant difference because the energy transfer from phycobilisome was blocked in powder.
Key words:  Te(IV) stress  Spirulina maxima  spectra properties
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