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深海芽孢杆菌E401B03 次生代谢产物分离和结构鉴定
郭书举¹, 史大永¹, 李富超¹, 朱校斌¹, 缪宇平²
1.中国科学院海洋研究所;2.中国水产科学院东海水产研究所

摘要:

利用硅胶柱、凝胶柱及高效液相(HPLC)等色谱方法, 对采自中国南海的深海芽孢杆菌E401B03发酵液的乙酸乙酯相浸膏进行了分离, 通过¹H-NMR、¹³C-NMR、EIMS 等方法鉴定了7 个化合物: N-苯乙基乙酰胺、3-(羟基乙酰基)-吲哚、3-(2-羟基乙基)-吲哚、环(缬-缬)二肽、尿嘧啶、胸腺嘧啶、对羟基苯甲醛。

关键词: 深海芽孢杆菌次生代谢产物

DOI：

分类号:

基金项目:农业部海洋与河口渔业资源及生态重点开放实验室开放课题(开-09-03); 江苏省自然科学基金项目(BK2011395)

Isolation and identification of secondary metabolites produced by a deep-sea Bacillus sp. E401B03

Abstract:

Seven compounds, were isolated from the ethyl acetate extract of Bacillus sp. E401B03. Compounds 1-7 were obtained by normal phase silica gel, Sephadex LH-20 chromatography and reverse phase HPLC techniques. Their structures were identified by spectroscopic methods including ¹H-NMR,¹³C-NMR, and EIMS.

Key words: deep-sea Bacillus sp. secondary metabolites

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