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凡纳滨对虾肠道微生物宏基因组Solexa 测序及其初步分析
严雪平1,2, 袁剑波1,2, 刘 斌1
1.中国科学院 海洋研究所 实验海洋生物学重点实验室;2.中国科学院 研究生院
摘要:
CTAB-酚/氯仿法提取新鲜凡纳滨对虾(Litopenaeus vannamei)肠道微生物宏基因组DNA。结果表明: CTAB-酚/氯仿法提取总DNA 的浓度达到92.5 ng/μL, 半定量PCR 法检测微生物基因组相对含量为69.9%。凡纳滨对虾肠道微生物宏基因组用于Solexa 测序, 生物信息学分析结果显示宏基因数据中64.1%的数据属于未知序列, 35.5%属于真核生物, 而已知的微生物和病毒序列所占比例仅有0.4%。
关键词:  肠道微生物宏基因组  CTAB  Solexa 测序
DOI：
分类号:
基金项目:国家高技术研究发展计划(2007AA09Z444)
Solexa sequencing and analysis of the intestinal microorganisms metagenome in Litopenaeus vannamei
Abstract:
The intestinal microorganisms metagenome of Litopenaeus vannamei was extracted by CTAB-phenol/ chloroform DNA extraction protocol. The relative concentration of bacterial genome in metagenome was analyzed by semi-quantitative PCR. The results showed that the total concentration of metagenome was 92.5 mg/L and relative microbial metagenome ratio was 69.9%. Solexa sequencing showed that up to 64.1% of contigs in metagenome had no assignment or no hits in database of NCBI, 35.5% of which were assigned to eukaryon, and only 0.4% sequences belonged to bacteria and virus.
Key words:  intestinal microfloar metagenome  CTAB  solexa sequencing
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